Western Blot (WB)
(Western blot analysis of lysate from 293T cell line, using CHCHD3 Antibody (Center). MBS9210872 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Rabbit anti-Human CHCHD3 Polyclonal Antibody | anti-CHCHD3 antibody
CHCHD3 Antibody (Center)
Gene Names
CHCHD3; Mic19; MINOS3; PPP1R22
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CHCHD3; Polyclonal Antibody; CHCHD3 Antibody (Center); MICOS complex subunit MIC19; Coiled-coil-helix-coiled-coil-helix domain-containing protein 3; MIC19; MINOS3; anti-CHCHD3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CHCHD3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 83-116 amino acids from the Central region of human CHCHD3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
227
Applicable Applications for anti-CHCHD3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysate from 293T cell line, using CHCHD3 Antibody (Center). MBS9210872 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from 293T cell line, using CHCHD3 Antibody (Center). MBS9210872 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-CHCHD3 antibody
Required for maintenance of mitochondrial crista integrity and mitochondrial function. May act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription.
Product Categories/Family for anti-CHCHD3 antibody
References
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Daub H.,et al.Mol. Cell 31:438-448(2008).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Mayya V.,et al.Sci. Signal. 2:RA46-RA46(2009).
Choudhary C.,et al.Science 325:834-840(2009).
Daub H.,et al.Mol. Cell 31:438-448(2008).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Mayya V.,et al.Sci. Signal. 2:RA46-RA46(2009).
Choudhary C.,et al.Science 325:834-840(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26152
NCBI Official Full Name
MICOS complex subunit MIC19
NCBI Official Synonym Full Names
coiled-coil-helix-coiled-coil-helix domain containing 3
NCBI Official Symbol
CHCHD3
NCBI Official Synonym Symbols
Mic19; MINOS3; PPP1R22
NCBI Protein Information
MICOS complex subunit MIC19
UniProt Protein Name
MICOS complex subunit MIC19
UniProt Gene Name
CHCHD3
UniProt Synonym Gene Names
MIC19; MINOS3
UniProt Entry Name
MIC19_HUMAN
Uniprot Description
CHCHD3: May be a scaffolding protein that stabilizes protein complexes involved in maintaining mitochondrial crista architecture and protein import.
Protein type: Mitochondrial
Chromosomal Location of Human Ortholog: 7q33
Cellular Component: mitochondrion; mitochondrial inner membrane; cytoplasm; nucleus
Molecular Function: protein binding; protein complex scaffold; phosphatase binding
Biological Process: inner mitochondrial membrane organization and biogenesis; mitochondrial fusion; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
Research Articles on CHCHD3
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Product Notes
The CHCHD3 chchd3 (Catalog #AAA9210872) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHCHD3 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHCHD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the CHCHD3 chchd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHCHD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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