Rabbit CSNK1E Polyclonal Antibody | anti-CSNK1E antibody

CSNK1E antibody

Cat. Num. AAA9411738

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: CSNK1E; Polyclonal Antibody; CSNK1E antibody; HCKIE; CKIepsilon; anti-CSNK1E antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total CSNK1E antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 416

• Applicable Applications for anti-CSNK1E antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human CSNK1E.
• Target Name: CSNK1E
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CSNK1E antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using CSNK1E antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-CSNK1E antibody

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-CSNK1E antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 583966087
• NCBI GeneID: 102800317
• NCBI Accession #: NP_001276841.1
• NCBI GenBank Nucleotide #: NM_001289912.1
• UniProt Accession #: P49674
• Molecular Weight: 47,315 Da
• NCBI Official Full Name: casein kinase I isoform epsilon
• NCBI Official Symbol: LOC400927-CSNK1E
• NCBI Protein Information: casein kinase I isoform epsilon
• UniProt Protein Name: Casein kinase I isoform epsilon
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK1E
• UniProt Synonym Gene Names: CKI-epsilon; CKIe
• UniProt Entry Name: KC1E_HUMAN

NCBI Description

This locus represents naturally occurring readthrough transcription between the neighboring LOC400927 (transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 pseudogene) and CSNK1E (casein kinase I isoform epsilon) genes on chromosome 22. The readthrough transcript encodes the same protein as the downstream gene product (casein kinase I isoform epsilon). [provided by RefSeq, Feb 2014]

Uniprot Description

CK1E: an ubiquitous protein kinase of the CK1 family. Together with Dvl-1 and Frat-1 activate the Wnt signaling pathway. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. May play a role in cell cycle progression. Two splice variant isoforms have been described. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, BMAL1 or BMAL2, CK1-D and/or CK1-E, TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 which may lead to their degradation. Interacts with SOCS3. Mutations in hamster and Drosophila orthologs have circadian rhythm phenotypes, and the circadian gene period (per) is a substrate in both human and fly. A coding SNP variant in human, which increases CK1 activity, is negatively associated with circadian disorder. LOF mutations and LOH seen in mammary ductal carcinoma.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CK1; Kinase, protein; CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; cytosol; ribonucleoprotein complex; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: circadian rhythm; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; organelle organization and biogenesis; endocytosis; signal transduction; regulation of circadian rhythm; DNA repair; protein amino acid phosphorylation; regulation of cell shape; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; circadian regulation of gene expression; G2/M transition of mitotic cell cycle