Western Blot (WB)
(Western blot analysis of extracts from Mouse heart tissue, using phospho-CFL1/CFL2-Y88 antibody)
Rabbit anti-Human, Mouse CFL1/CFL2 Polyclonal Antibody | anti-CFL1/CFL2 antibody
CFL1/CFL2, Phosphorylated (Y88) (Cofilin 1/2) (HRP)
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
CFL1/CFL2; Polyclonal Antibody; Phosphorylated (Y88) (Cofilin 1/2) (HRP); anti-CFL1/CFL2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes CFL1/CFL2, phosphorylated Y88. Species Crossreactivity: human, mouse
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Concentration
~1mg/ml (varies by lot)
Sequence Length
166
Applicable Applications for anti-CFL1/CFL2 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
WB: 1:500-1:1000
Applications are based on unconjugated antibody.
WB: 1:500-1:1000
Applications are based on unconjugated antibody.
Immunogen
Phospho specific peptide corresponding to residues surrounding Y88 of human CFL1/CFL2.
Conjugate
HRP
Antigen Modification
Phosphorylated
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of extracts from Mouse heart tissue, using phospho-CFL1/CFL2-Y88 antibody)
Western Blot (WB)
(Western blot analysis of extracts from Mouse heart tissue, using phospho-CFL1/CFL2-Y88 antibody)
Product Categories/Family for anti-CFL1/CFL2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
19
NCBI Official Full Name
Cofilin-1
NCBI Official Synonym Full Names
cofilin 1
NCBI Official Symbol
CFL1
NCBI Official Synonym Symbols
CFL; cofilin; HEL-S-15
NCBI Protein Information
cofilin-1
NCBI Description
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
Research Articles on CFL1/CFL2
Similar Products
Product Notes
The CFL1/CFL2 (Catalog #AAA6411375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CFL1/CFL2, Phosphorylated (Y88) (Cofilin 1/2) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CFL1/CFL2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:100 WB: 1:500-1:1000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CFL1/CFL2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CFL1/CFL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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