Rabbit anti-Mouse Abl2 Polyclonal Antibody | anti-Abl2 antibody

Mouse Abl2 Antibody (C-term)

Cat. Num. AAA9216252

• Gene Names: Abl2; Arg; Abll; AA536808
• Reactivity: Mouse
• Applications: Western Blot
• Synonyms: Abl2; Polyclonal Antibody; Mouse Abl2 Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab); Abelson tyrosine-protein kinase 2; Abelson murine leukemia viral oncogene homolog 2; Abelson-related gene protein; Tyrosine-protein kinase ARG; Abl2 {ECO:0000312|EMBL:AAY860391; ECO:0000312|MGI:MGI:87860}; anti-Abl2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This Mouse Abl2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1011-1044 amino acids from the C-terminal region of Mouse Abl2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 1182

• Applicable Applications for anti-Abl2 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-Abl2 Antibody (C-term)at 1:2000 dilution + mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 128 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Abl2 antibody

Non-receptor tyrosine-protein kinase that plays an ABL1- overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL or DOK1. Required for adhesion-dependent phosphorylation of ARHGAP35 which promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

Product Categories/Family for anti-Abl2 antibody

Cancer; Signal Transduction

References

Wang Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 98:14865-14870(2001). Tanis K.Q.,et al.Mol. Cell. Biol. 23:3884-3896(2003). Koleske A.J.,et al.Neuron 21:1259-1272(1998). Kain K.H.,et al.J. Biol. Chem. 276:16185-16192(2001). Woodring P.J.,et al.J. Cell Sci. 116:2613-2626(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 118582158
• NCBI GeneID: 11352
• UniProt Accession #: Q4JIM5
• Molecular Weight: 128196
• NCBI Official Full Name: Abelson tyrosine-protein kinase 2
• NCBI Official Synonym Full Names: v-abl Abelson murine leukemia viral oncogene 2 (arg, Abelson-related gene)
• NCBI Official Symbol: Abl2
• NCBI Official Synonym Symbols: Arg; Abll; AA536808
• NCBI Protein Information: Abelson tyrosine-protein kinase 2
• UniProt Protein Name: Abelson tyrosine-protein kinase 2
• Protein Family: Abelson tyrosine-protein kinase
• UniProt Gene Name: Abl2
• UniProt Entry Name: ABL2_MOUSE

NCBI Description

Arg: a cytoplasmic tyrosine kinase of the Abl family. Expressed at the highest levels in mature B cells. Promotes catalase degradation in the oxidative stress response. Localizes to dynamic actin structures and may regulate cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Oncoprotein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Abl family

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; lamellipodium; cytoplasmic membrane-bound vesicle; cytoplasm; dendritic spine; actin cytoskeleton; phagocytic cup

Molecular Function: transferase activity; protein binding; manganese ion binding; metal ion binding; protein-tyrosine kinase activity; nucleotide binding; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; actin filament bundle formation; cell migration; peptidyl-tyrosine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein binding; dendrite morphogenesis; actin filament organization; alpha-beta T cell differentiation; cerebellum morphogenesis; platelet-derived growth factor receptor signaling pathway; learning; substrate-bound cell migration, cell extension; protein amino acid phosphorylation; phagocytosis; regulation of cell proliferation; Bergmann glial cell differentiation; elevation of cytosolic calcium ion concentration; negative regulation of Rho protein signal transduction; innate immune response; visual learning; negative regulation of cell-cell adhesion; cell adhesion; neuromuscular process controlling balance; actin cytoskeleton organization and biogenesis; phosphorylation; positive regulation of oxidoreductase activity; neuron remodeling

Uniprot Description

Arg: a cytoplasmic tyrosine kinase of the Abl family. Expressed at the highest levels in mature B cells. Promotes catalase degradation in the oxidative stress response. Localizes to dynamic actin structures and may regulate cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Abl family

Cellular Component: actin cytoskeleton; cytoplasm; cytoplasmic membrane-bound vesicle; cytoskeleton; dendritic spine; extrinsic to internal side of plasma membrane; lamellipodium; phagocytic cup

Molecular Function: ATP binding; kinase activity; magnesium ion binding; manganese ion binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; transferase activity

Biological Process: actin cytoskeleton organization and biogenesis; actin filament bundle formation; actin filament organization; alpha-beta T cell differentiation; Bergmann glial cell differentiation; cell adhesion; cerebellum morphogenesis; dendrite morphogenesis; elevation of cytosolic calcium ion concentration; epidermal growth factor receptor signaling pathway; innate immune response; learning; negative regulation of cell-cell adhesion; negative regulation of Rho protein signal transduction; neuromuscular process controlling balance; neuron remodeling; peptidyl-tyrosine phosphorylation; phagocytosis; phosphorylation; platelet-derived growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of oxidoreductase activity; positive regulation of protein binding; protein amino acid phosphorylation; regulation of cell proliferation; substrate-bound cell migration, cell extension; visual learning