Rabbit anti-Human, Mouse CF153 Polyclonal Antibody | anti-CF153 antibody

CF153, CT (RRP36, Ribosomal RNA processing protein 36 homolog) (PE)

Cat. Num. AAA6285336

• Gene Names: RRP36; C6orf153; dJ20C7.4
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CF153; Polyclonal Antibody; CT (RRP36; Ribosomal RNA processing protein 36 homolog) (PE); RRP36; Ribosomal RNA processing protein 36 homolog; anti-CF153 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CF153 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CF153 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-263 amino acids from the C-terminal region of human CF153.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CF153 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the CF153 antibody detected the CF153 protein (arrow).)

Immunohistochemistry (IHC)

(CF153 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CF153 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CF153 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CF153 antibody

RRP36 functions at an early stage in the processing of 35S preribosomal RNA into the mature 18S species (Gerus et al., 2010 [PubMed 20038530]).

Product Categories/Family for anti-CF153 antibody

Antibodies; Ribosomal Proteins

References

Gerus, M., et al. Mol. Cell. Biol. 30(5):1130-1144(2010). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Comtesse, N., et al. Proc. Natl. Acad. Sci. U.S.A. 102(27):9601-9606(2005). Andersen, J.S., et al. Nature 433(7021):77-83(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 24308350
• NCBI GeneID: 88745
• NCBI Accession #: NP_149103.1
• NCBI GenBank Nucleotide #: NM_033112.2
• UniProt Accession #: Q96EU6; Q9BRF6; Q9P0C8
• Molecular Weight: 29,823 Da
• NCBI Official Full Name: ribosomal RNA processing protein 36 homolog
• NCBI Official Synonym Full Names: ribosomal RNA processing 36 homolog (S. cerevisiae)
• NCBI Official Symbol: RRP36
• NCBI Official Synonym Symbols: C6orf153; dJ20C7.4
• NCBI Protein Information: ribosomal RNA processing protein 36 homolog; RNA processing factor
• UniProt Protein Name: Ribosomal RNA processing protein 36 homolog
• UniProt Gene Name: RRP36
• UniProt Synonym Gene Names: C6orf153; HSPC253
• UniProt Entry Name: RRP36_HUMAN