Rabbit anti-Human CES2 Polyclonal Antibody | anti-CES2 antibody

CES2, ID (CES2, ICE, Cocaine esterase, Carboxylesterase 2, Methylumbelliferyl-acetate deacetylase 2) (FITC)

Cat. Num. AAA6285252

• Gene Names: CES2; iCE; CE-2; PCE-2; CES2A1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CES2; Polyclonal Antibody; ID (CES2; ICE; Cocaine esterase; Carboxylesterase 2; Methylumbelliferyl-acetate deacetylase 2) (FITC); Methylumbelliferyl-acetate deacetylase 2; anti-CES2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CES2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CES2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 340-369 in the central region of human CES2, UniProt Accession #NP_003860; O00748.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(All lanes: MBS647331 (1:2000) Lane 1: HepG2 whole cell lysates (20ug/lane) Lane 2: human liver lysates (20ug/lane). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000. Predicted band size: 62kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(All lanes: MBS647331 (1:2000) Lane 1: SW620 whole cell lysates (20ug/lane) Lane 2: human liver lysates (20ug/lane). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000. Predicted band size: 62kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot analysis in HepG2 whole cell lysates (20ug/lane) using MBS647331 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 62kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using MBS647331 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS647331 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric overlay histogram showing U-87 MG cells stained with MBS647331 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% BSA to block non-specific protein-protein interactions followed by033793 (1:25) for 60 min at 37 degree C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) at 1:400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

Related Product Information for anti-CES2 antibody

CES2 is a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance.

Product Categories/Family for anti-CES2 antibody

Antibodies; Enzymes

References

1. Holmes, R.S., et al. Mamm. Genome 21 (9-10), 427-441 (2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. Howard, T.D., et al. Environ. Health Perspect. 118(10):1395-1399(2010). 4. Hatfield, M.J., et al. Br. J. Pharmacol. 160(8):1916-1928(2010). 5. Holmes, R.S., et al. Genetica 138(7):695-708(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 37622885
• NCBI GeneID: 8824
• NCBI Accession #: NP_003860
• NCBI GenBank Nucleotide #: NM_003869
• UniProt Accession #: O00748; Q16859; Q5MAB8; Q7Z366; Q8IUP4; Q8TCP8; A8K367
• Molecular Weight: 59,959 Da
• NCBI Official Full Name: cocaine esterase isoform 1
• NCBI Official Synonym Full Names: carboxylesterase 2
• NCBI Official Symbol: CES2
• NCBI Official Synonym Symbols: iCE; CE-2; PCE-2; CES2A1
• NCBI Protein Information: cocaine esterase; carboxylesterase 2 (intestine, liver); hCE-2; intestinal carboxylesterase; liver carboxylesterase-2; methylumbelliferyl-acetate deacetylase 2
• UniProt Protein Name: Cocaine esterase
• Protein Family: Liver carboxylesterase
• UniProt Gene Name: CES2
• UniProt Synonym Gene Names: ICE; CE-2; hCE-2
• UniProt Entry Name: EST2_HUMAN

Uniprot Description

CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.56; EC 3.1.1.84; Xenobiotic Metabolism - drug metabolism - other enzymes; Endoplasmic reticulum; EC 3.1.1.1; Hydrolase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space; endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: methylumbelliferyl-acetate deacetylase activity

Biological Process: catabolic process