Rabbit anti-Human MRPS11 Polyclonal Antibody | anti-MRPS11 antibody

MRPS11, ID (MRPS11, RPMS11, 28S ribosomal protein S11, mitochondrial, Cervical cancer proto-oncogene 2 protein) (Biotin)

Cat. Num. AAA6322276

• Gene Names: MRPS11; HCC-2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MRPS11; Polyclonal Antibody; ID (MRPS11; RPMS11; 28S ribosomal protein S11; mitochondrial; Cervical cancer proto-oncogene 2 protein) (Biotin); Cervical cancer proto-oncogene 2 protein; anti-MRPS11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MRPS11 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MRPS11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-69 amino acids from the Central region of human MRPS11.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MRPS11 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MRPS11 antibody detected the MRPS11 protein (arrow).)

Related Product Information for anti-MRPS11 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that contains a high level of sequence similarity with ribosomal protein S11P family members. A pseudogene corresponding to this gene is found on chromosome 20. Sequence analysis identified two transcript variants that encode different protein isoforms.

Product Categories/Family for anti-MRPS11 antibody

Antibodies; Cancer Markers

References

Ishiguchi, H., et al. Int. J. Cancer 111(6):900-909(2004). Zhang, Z., et al. Genomics 81(5):468-480(2003). Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001): Suzuki, T., et al. J. Biol. Chem. 276(35):33181-33195(2001). Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 16554609
• NCBI GeneID: 64963
• NCBI Accession #: NP_073750.2; NP_789775.1
• NCBI GenBank Nucleotide #: NM_022839.3
• UniProt Accession #: P82912; Q969D7; Q96GI3; Q9BYC3; B2RD52
• Molecular Weight: 16,904 Da
• NCBI Official Full Name: 28S ribosomal protein S11, mitochondrial isoform a
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S11
• NCBI Official Symbol: MRPS11
• NCBI Official Synonym Symbols: HCC-2
• NCBI Protein Information: 28S ribosomal protein S11, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S11, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: MRPS11
• UniProt Synonym Gene Names: RPMS11; MRP-S11; S11mt; HCC-2
• UniProt Entry Name: RT11_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that contains a high level of sequence similarity with ribosomal protein S11P family members. A pseudogene corresponding to this gene is found on chromosome 20. Sequence analysis identified two transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS11: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that contains a high level of sequence similarity with ribosomal protein S11P family members. A pseudogene corresponding to this gene is found on chromosome 20. Sequence analysis identified two transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; RNA-binding; Mitochondrial; Translation

Chromosomal Location of Human Ortholog: 15q25

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial small ribosomal subunit

Molecular Function: structural constituent of ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis; DNA damage response, detection of DNA damage