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Western Blot (WB)
(Western blot analysis of CEP110 using COS7 whole cell lysates)
Rabbit CEP110 Polyclonal Antibody | anti-CEP110 antibody
CEP110 Antibody
Gene Names
CCP110; CP110; Cep110
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CEP110; Polyclonal Antibody; CEP110 Antibody; 110 kDa centrosomal protein; CCP110; Centriolar coiled coil protein 110kDa; Centrosomal protein 110 kDa; Centrosomal protein CP110; Centrosomal protein of 110 kDa; Cep110; CP110; CP110_HUMAN; DKFZp781G1416; FAN; KIAA0419; anti-CEP110 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CEP110 antibody detects endogenous levels of total CEP110
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1012
Applicable Applications for anti-CEP110 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human CEP110
Subcellular Location
Cytoplasm > Cytoskeleton > Centrosome > Centriole. Recruited early and then associates with the growing distal tips.
Tissue Specificity
Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (90%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of CEP110 using COS7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of CEP110 using COS7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of extracts from rat brain, using CEP110 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from rat brain, using CEP110 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse lung, using CEP110 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse lung, using CEP110 Antibody.)
Immunohistochemistry (IHC)
(MBS9609677 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609677 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CEP110 antibody
Function: Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation (PubMed:17719545 PubMed:17681131, PubMed:23486064). Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation (By similarity). Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2 (PubMed:16760425).
Subunit Structure: Interacts with CALM1, CETN2, CEP76, CEP97, CEP104, CEP290, TALPID3. Seems to associate with discrete CETN2, CEP97 and CEP290-containing complexes. Interacts with NEURL4 and CCNF; these interactions are not mutually exclusive and both lead to CCP110 ubiquitination and proteasome-dependent degradation. Via its interaction with NEURL4, may indirectly interact with HERC2. Interacts with KIF24, leading to its recruitment to centrioles. Interacts with USP20 and USP33.
Post-translational Modifications: Phosphorylated by CDKs. Ubiquitinated by the SCF(CCNF) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication. Deubiquitinated by USP33 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate.
Subunit Structure: Interacts with CALM1, CETN2, CEP76, CEP97, CEP104, CEP290, TALPID3. Seems to associate with discrete CETN2, CEP97 and CEP290-containing complexes. Interacts with NEURL4 and CCNF; these interactions are not mutually exclusive and both lead to CCP110 ubiquitination and proteasome-dependent degradation. Via its interaction with NEURL4, may indirectly interact with HERC2. Interacts with KIF24, leading to its recruitment to centrioles. Interacts with USP20 and USP33.
Post-translational Modifications: Phosphorylated by CDKs. Ubiquitinated by the SCF(CCNF) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication. Deubiquitinated by USP33 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 113 kDa
Predicted: 114 kDa
Predicted: 114 kDa
NCBI Official Full Name
centriolar coiled-coil protein of 110 kDa isoform 1
NCBI Official Synonym Full Names
centriolar coiled-coil protein 110
NCBI Official Symbol
CCP110
NCBI Official Synonym Symbols
CP110; Cep110
NCBI Protein Information
centriolar coiled-coil protein of 110 kDa
UniProt Protein Name
Centriolar coiled-coil protein of 110 kDa
UniProt Gene Name
CCP110
UniProt Synonym Gene Names
CEP110; CP110; KIAA0419; CP110; Cep110
Uniprot Description
Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation (PubMed:17719545 PubMed:17681131, PubMed:23486064). Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation (). Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2 (PubMed:16760425).
Research Articles on CEP110
Similar Products
Product Notes
The CEP110 ccp110 (Catalog #AAA9609677) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CEP110 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CEP110 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the CEP110 ccp110 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEP110, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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