Rabbit anti-Human Centaurin delta 1 Polyclonal Antibody | anti-ARAP2 antibody

Centaurin delta 1 (Centaurin-delta-1, CENTD1, Cnt-d1, Arf-GAP with Rho-GAP Domain ANK Repeat and PH Domain-containing Protein 2, ARAP2, KIAA0580, PARX Protein)

Cat. Num. AAA643935

• Gene Names: ARAP2; PARX; CENTD1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Centaurin delta 1; Polyclonal Antibody; Centaurin delta 1 (Centaurin-delta-1; CENTD1; Cnt-d1; Arf-GAP with Rho-GAP Domain ANK Repeat and PH Domain-containing Protein 2; ARAP2; KIAA0580; PARX Protein); Anti -Centaurin delta 1 (Centaurin-delta-1; anti-ARAP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ARAP2. Species sequence homology: chimpanzee, bovine, equine, monkey.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

• Applicable Applications for anti-ARAP2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to aa1670-1720 of human ARAP2.
• Positive Control: Human kidney lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of ARAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and A3334-96A (bottom left, right) at 5ug/ml.)

Western Blot (WB)

(Western blot analysis of ARAP2 in human kidney lysate in the 1) absence and 2) presence of immunizing peptide using A3334-96A at 1ug/ml. goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)

Related Product Information for anti-ARAP2 antibody

ARAP2 also known as Centaurin-delta-1 is a protein with ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. A phosphatidylinositol-3, 4, 5-trisphosphate-dependent GTPase-activating protein, ARAP2, binds to RhoA-GTP, but lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. It modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Usually ARAP2 is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding and can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. ARAP2 associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics. It is usually detected in brain, thymus, lymph node, thyroid, spinal cord, trachea, heart, skeletal muscle, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes.

Product Categories/Family for anti-ARAP2 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 119613278
• NCBI GeneID: 116984
• UniProt Accession #: Q8WZ64; Q4W5D2; Q7Z2L5; Q96L70; Q96P49; Q9Y4E4
• Molecular Weight: 193,452 Da
• NCBI Official Full Name: centaurin, delta 1, isoform CRA_c
• NCBI Official Synonym Full Names: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
• NCBI Official Symbol: ARAP2
• NCBI Official Synonym Symbols: PARX; CENTD1
• NCBI Protein Information: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2; cnt-d1; centaurin-delta-1; centaurin, delta 1; Arf and Rho GAP adapter protein 2
• UniProt Protein Name: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
• Protein Family: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein
• UniProt Gene Name: ARAP2
• UniProt Synonym Gene Names: CENTD1; KIAA0580; Cnt-d1
• UniProt Entry Name: ARAP2_HUMAN

NCBI Description

The protein encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. The protein is a phosphatidylinositol (3,4,5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP, but it lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. The protein associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics. [provided by RefSeq, Sep 2008]

Uniprot Description

CENTD1: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency.

Protein type: GAPs; GAPs, ARF

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: cytosol

Molecular Function: phosphatidylinositol-3,4,5-triphosphate binding; zinc ion binding

Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity