Rabbit anti-Human CEBPB Polyclonal Antibody | anti-CEBPB antibody

CEBPB, CT (CCAAT/Enhancer-binding Protein beta, C/EBP beta, Liver Activator Protein, Nuclear factor NF-IL6, Transcription factor 5, PP9092, TCF5, LAP) (PE)

Cat. Num. AAA6468554

• Gene Names: CEBPB; TCF5; IL6DBP; NF-IL6; C/EBP-beta
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CEBPB; Polyclonal Antibody; CT (CCAAT/Enhancer-binding Protein beta; C/EBP beta; Liver Activator Protein; Nuclear factor NF-IL6; Transcription factor 5; PP9092; TCF5; LAP) (PE); anti-CEBPB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CEBPB.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CEBPB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CEBPB (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CEBPB (arrow) using rabbit polyclonal CEBPB Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CEBPB gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CEBPB Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CEBPB Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CEBPB antibody

Antibodies; Transcription Factors

References

1. Buck, M., et.al., Mol. Cell 4(6), 1087-1092 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 28872796
• NCBI GeneID: 1051
• NCBI Accession #: NP_005185
• NCBI GenBank Nucleotide #: NM_005194.3
• UniProt Accession #: P17676; Q96IH2; Q9H4Z5; A8K671
• Molecular Weight: 15,938 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein beta isoform a
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein beta
• NCBI Official Symbol: CEBPB
• NCBI Official Synonym Symbols: TCF5; IL6DBP; NF-IL6; C/EBP-beta
• NCBI Protein Information: CCAAT/enhancer-binding protein beta
• UniProt Protein Name: CCAAT/enhancer-binding protein beta
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: CEBPB
• UniProt Synonym Gene Names: LAP; TCF5; C/EBP beta; TCF-5
• UniProt Entry Name: CEBPB_HUMAN

NCBI Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013]

Uniprot Description

C/EBP-beta: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-alpha, -delta and -gamma. Involved in immune and inflammatory responses. Specifically binds to regulatory regions of genes encoding IL-6, other cytokines and several acute-phase proteins. There are two forms of C/EBPbeta, the 38kDa liver activating protein (LAP) and the 20kDa liver inhibitory protein (LIP) which may be products of alternative translation. LAP is a transcriptional activator while LIP may inhibit C/EBPbeta transcriptional activity. Phosphorylated and activated by ERK1/2.

Protein type: Motility/polarity/chemotaxis; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm; nuclear matrix; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; chromatin binding; transcription factor activity; transcription factor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; regulation of interleukin-6 biosynthetic process; mammary gland epithelial cell proliferation; response to lipopolysaccharide; neuron differentiation; positive regulation of osteoblast differentiation; regulation of transcription, DNA-dependent; brown fat cell differentiation; acute-phase response; positive regulation of fat cell differentiation; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; inflammatory response; negative regulation of transcription, DNA-dependent