Mouse Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit | Smad1 elisa kit

Mouse Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit

Cat. Num. AAA2088137

• Gene Names: Smad1; Mlp1; Madh1; Madr1; MusMLP; AI528653
• Reactivity: Mouse
• Synonyms: Mothers Against Decapentaplegic Homolog 1 (Smad1); Mouse Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit; Smad1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mothers Against Decapentaplegic Homolog 1 (Smad1).; No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 1 (Smad1) and analogues was observed.
• Sequence Length: 465

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.125ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 1 (Smad1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 1 (Smad1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Smad1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 1 (Smad1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 1 (Smad1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 1 (Smad1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 1 (Smad1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for Smad1 elisa kit

Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 31543220
• NCBI GeneID: 17125
• NCBI Accession #: NP_032565.2
• NCBI GenBank Nucleotide #: NM_008539.3
• UniProt Accession #: P70340; P70442; Q6GT95; Q9CYK6
• Molecular Weight: 52,157 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 1
• NCBI Official Synonym Full Names: SMAD family member 1
• NCBI Official Symbol: Smad1
• NCBI Official Synonym Symbols: Mlp1; Madh1; Madr1; MusMLP; AI528653
• NCBI Protein Information: mothers against decapentaplegic homolog 1
• UniProt Protein Name: Mothers against decapentaplegic homolog 1
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: Smad1
• UniProt Synonym Gene Names: Madh1; Madr1; MAD homolog 1; Mothers against DPP homolog 1; Dwf-A; Mad-related protein 1; mMad1; SMAD 1; Smad1

Uniprot Description

SMAD1: transcription factor phosphorylated and activated by bone morphogenetic protein type 1 receptor kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated forms dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 8|8 C1

Cellular Component: cytoplasm; nuclear inner membrane; nucleus; protein complex; transcription factor complex

Molecular Function: identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; transcription factor activity

Biological Process: BMP signaling pathway; cardiac muscle cell proliferation; cartilage development; gamete generation; hindbrain development; homeostatic process; inflammatory response; MAPKKK cascade; mesodermal cell fate commitment; midbrain development; negative regulation of cell proliferation; osteoblast fate commitment; positive regulation of cell differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of transcription from RNA polymerase II promoter; response to drug; SMAD protein complex assembly; ureteric bud development