Western Blot (WB)
(Figure 1. Western blot analysis of CEBP Delta/CEBPD using anti-CEBP Delta/CEBPD antibody (MBS1753600).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 whole cell lysatesLane 2: human placenta tissue lysatesLane 3: human U87 whole cell lysatesLane 4: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CEBP Delta/CEBPD antigen affinity purified polyclonal antibody (Catalog # MBS1753600) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for CEBP Delta/CEBPD at approximately 36KD. The expected band size for CEBP Delta/CEBPD is at 36KD. )
Rabbit anti-Human, Mouse CEBP Delta/CEBPD Polyclonal Antibody | anti-CEBPD antibody
Anti-CEBP Delta/CEBPD Antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of CEBP Delta/CEBPD using anti-CEBP Delta/CEBPD antibody (MBS1753600).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 whole cell lysatesLane 2: human placenta tissue lysatesLane 3: human U87 whole cell lysatesLane 4: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CEBP Delta/CEBPD antigen affinity purified polyclonal antibody (Catalog # MBS1753600) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for CEBP Delta/CEBPD at approximately 36KD. The expected band size for CEBP Delta/CEBPD is at 36KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of CEBP Delta/CEBPD using anti-CEBP Delta/CEBPD antibody (MBS1753600).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 whole cell lysatesLane 2: human placenta tissue lysatesLane 3: human U87 whole cell lysatesLane 4: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CEBP Delta/CEBPD antigen affinity purified polyclonal antibody (Catalog # MBS1753600) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for CEBP Delta/CEBPD at approximately 36KD. The expected band size for CEBP Delta/CEBPD is at 36KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-CEBP Delta/CEBPD antibody (MBS1753600).Overlay histogram showing SiHa cells stained with MBS1753600 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBP Delta/CEBPD Antibody (MBS1753600, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-CEBP Delta/CEBPD antibody (MBS1753600).Overlay histogram showing SiHa cells stained with MBS1753600 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBP Delta/CEBPD Antibody (MBS1753600, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Cleutjens, C. B. J. M., van Eekelen, C. C. E. M., van Dekken, H., Smit, E. M. E., Hagemeijer, A., Wagner, M. J., Wells, D. E., Trapman, J. The human C/EBP-delta (CRP3/CELF) gene: structure and chromosomal localization. Genomics 16: 520-523, 1993.
3. Gigliotti, A. P., Johnson, P. F., Sterneck, E., DeWille, J. W. Nulliparous CCAAT/enhancer binding protein-delta (C/EBP-delta) knockout mice exhibit mammary gland ductal hyperlasia (sic). Exp. Biol. Med. 228: 278-285, 2003.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses, and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. The cytogenetic location of this locus has been reported as both 8p11 and 8q11. [provided by RefSeq, Sep 2010]
Uniprot Description
C/EBP-delta: C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses, may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha. Interacts with SPI1/PU.1. Interacts with PRDM16. Belongs to the bZIP family. C/EBP subfamily.
Protein type: DNA-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 8p11.2-p11.1
Cellular Component: nucleus
Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter
Research Articles on CEBPD
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Product Notes
The CEBPD cebpd (Catalog #AAA1753600) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CEBP Delta/CEBPD Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CEBP Delta/CEBPD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the CEBPD cebpd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEBP Delta/CEBPD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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