Rabbit anti-Human CEBP alpha Polyclonal Antibody | anti-CEBPA antibody

CEBP alpha, phosphorylated (Ser21) (CCAAT Enhancer Binding Protein, alpha)

Cat. Num. AAA613619

• Gene Names: CEBPA; CEBP; C/EBP-alpha
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Protein A and peptide affinity chromatography
• Synonyms: CEBP alpha; Polyclonal Antibody; phosphorylated (Ser21) (CCAAT Enhancer Binding Protein; alpha); Anti -CEBP alpha; anti-CEBPA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of serine 21 phosphorylated C/EBPalpha. Does not crossreact with other phosphorylated C/EBP isoforms
• Purity/Purification: Affinity Purified; Protein A and peptide affinity chromatography
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol

• Applicable Applications for anti-CEBPA antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phospho- Ser21 peptide (KLH coupled) corresponding to residues surrounding serine 21 of human C/EBPalpha
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Western Blot (WB)

(Western blot analysis of extracts from mouse adipocytes treated with insulin for the indicated times, using MBS613619)

Western Blot (WB)

(Western blot analysis of extracts of COS cells untransfected (lane 1), or transfected with wild-type mouse C/EBPalpha (lane 2), S21A (lane 3), and S21D (lane 4) mutants, using MBS613619(upper) or C/EBPalpha Antibody (lower). (Provided by Dr. Hanna Radomska, Beth Israel Deaconess Medical Center, Boston, MA).)

Related Product Information for anti-CEBPA antibody

CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for

Product Categories/Family for anti-CEBPA antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 28872794
• NCBI GeneID: 1050
• NCBI Accession #: NP_004355.2
• NCBI GenBank Nucleotide #: NM_004364.3
• UniProt Accession #: P49715; P78319; Q05CA4; A7LNP2; Q6P3S4
• Molecular Weight: 37,561 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein alpha
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), alpha
• NCBI Official Symbol: CEBPA
• NCBI Official Synonym Symbols: CEBP; C/EBP-alpha
• NCBI Protein Information: CCAAT/enhancer-binding protein alpha; c/EBP alpha; OTTHUMP00000220269
• UniProt Protein Name: CCAAT/enhancer-binding protein alpha
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: CEBPA
• UniProt Entry Name: CEBPA_HUMAN

NCBI Description

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq]

Uniprot Description

C/EBP-alpha: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-beta and CEBP-gamma. Binds to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Rb-E2F complex; nuclear matrix; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; protein complex binding; transcription factor binding; transcription factor activity

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; embryonic placenta development; macrophage differentiation; viral reproduction; response to glucocorticoid stimulus; cell maturation; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; negative regulation of cell proliferation; response to vitamin B2; acute-phase response; inner ear development; mitochondrion organization and biogenesis; cholesterol metabolic process; organ regeneration; generation of precursor metabolites and energy; transcription, DNA-dependent; granulocyte differentiation; cytokine and chemokine mediated signaling pathway; negative regulation of cyclin-dependent protein kinase activity; liver development; positive regulation of osteoblast differentiation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; white fat cell differentiation; brown fat cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase III promoter; positive regulation of transcription from RNA polymerase II promoter; myeloid cell differentiation; urea cycle; lung development

Disease: Leukemia, Acute Myeloid