Western Blot (WB)
(Western blot analysis of CDKL5 using K562 whole cell lysates)
Rabbit anti-Human, Mouse CDKL5 Polyclonal Antibody | anti-CDKL5 antibody
CDKL5 Antibody
Gene Names
CDKL5; ISSX; STK9; EIEE2; CFAP247
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CDKL5; Polyclonal Antibody; CDKL5 Antibody; Cdkl5; CDKL5_HUMAN; Cyclin dependent kinase 5 transcript; Cyclin-dependent kinase-like 5; EIEE2; ISSX; Serine/threonine kinase 9; Serine/threonine-protein kinase 9; Stk9; anti-CDKL5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CDKL5 antibody detects endogenous levels of total CDKL5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1030
Applicable Applications for anti-CDKL5 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human CDKL5
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in brain, lung, kidney, prostate, ovary, placenta, pancreas and testis.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (92%), Bovine (92%), Horse (100%), Sheep (92%), Rabbit (100%), Dog (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of CDKL5 using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis of CDKL5 using K562 whole cell lysates)
Immunohistochemistry (IHC)
(MBS9609082 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609082 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CDKL5 antibody
Function: Mediates phosphorylation of MECP2 (PubMed:15917271, PubMed:16935860). May regulate ciliogenesis (PubMed:29420175).
Subunit Structure: Interacts with MECP2.
Post-translational Modifications: Autophosphorylated.
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Subunit Structure: Interacts with MECP2.
Post-translational Modifications: Autophosphorylated.
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 116 kDa
Predicted: 116 kDa
Predicted: 116 kDa
NCBI Official Full Name
cyclin-dependent kinase-like 5 isoform 1
NCBI Official Synonym Full Names
cyclin dependent kinase like 5
NCBI Official Symbol
CDKL5
NCBI Official Synonym Symbols
ISSX; STK9; EIEE2; CFAP247
NCBI Protein Information
cyclin-dependent kinase-like 5
UniProt Protein Name
Cyclin-dependent kinase-like 5
Protein Family
UniProt Gene Name
CDKL5
NCBI Description
This gene is a member of Ser/Thr protein kinase family and encodes a phosphorylated protein with protein kinase activity. Mutations in this gene have been associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
Mediates phosphorylation of MECP2 (PubMed:15917271, PubMed:16935860). May regulate ciliogenesis (PubMed:29420175).
Research Articles on CDKL5
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Product Notes
The CDKL5 cdkl5 (Catalog #AAA9609082) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDKL5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CDKL5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the CDKL5 cdkl5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDKL5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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