Rabbit Adiponectin Polyclonal Antibody | anti-ADIPOQ antibody

Rabbit Polyclonal to Human Adiponectin

Cat. Num. AAA240493

• Gene Names: ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: Adiponectin; Polyclonal Antibody; Rabbit Polyclonal to Human Adiponectin; Anti-Adiponectin Antibody (C-Terminus) IHC-plus; ADPN; ACDC; ADIPQTL1; APM-1; APM1; ACRP30; Gelatin-binding protein 28; GBP28; Gelatin-binding protein; Human Adiponectin; anti-ADIPOQ antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: 15 amino acid peptide from near the carboxy-terminus of human adiponectin
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 244

• Applicable Applications for anti-ADIPOQ antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (2.5 ug/ml), WB (1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human ADIPOQ / Adiponectin.
• Immunogen Type: Synthetic peptide
• Immunogen: Adiponectin antibody was raised against synthetic peptide from human ADIPOQ / Adiponectin.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-Adiponectin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of Adiponectin in Rat Brain cells with Adiponectin antibody at 10 ug/ml.)

Western Blot (WB)

(Western blot of adiponectin in HL60 cell lysate with adiponectin antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.)

Related Product Information for anti-ADIPOQ antibody

Adiponectin/APM1 is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. APM1 gene is exclusively expressed in adipose tissue. The protein, also known as gelatin-binding protein, has high similarity with collagens X and VIII and complement factor C1q. It is proposed that APM1 protein may be a collagen-like secretory protein.

NCBI and Uniprot Product Information

• NCBI GI #: 4757760
• NCBI GeneID: 9370
• NCBI Accession #: NP_004788.1
• NCBI GenBank Nucleotide #: NM_004797.3
• UniProt Accession #: Q15848; Q58EX9
• Molecular Weight: 26,414 Da
• NCBI Official Full Name: adiponectin
• NCBI Official Synonym Full Names: adiponectin, C1Q and collagen domain containing
• NCBI Official Symbol: ADIPOQ
• NCBI Official Synonym Symbols: ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
• NCBI Protein Information: adiponectin; gelatin-binding protein 28; adipose specific collagen-like factor; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipose most abundant gene transcript 1 protein
• UniProt Protein Name: Adiponectin
• Protein Family: Adiponectin
• UniProt Gene Name: ADIPOQ
• UniProt Synonym Gene Names: ACDC; ACRP30; APM1; GBP28; ACRP30; apM-1
• UniProt Entry Name: ADIPO_HUMAN

NCBI Description

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]

Uniprot Description

adiponectin: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex). Synthesized exclusively by adipocytes and secreted into plasma.

Protein type: Secreted; Hormone; Secreted, signal peptide; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space; cell surface; collagen; endoplasmic reticulum; extracellular region

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; hormone activity; cytokine activity; sialic acid binding; receptor binding

Biological Process: circadian rhythm; negative regulation of MAP kinase activity; negative regulation of phagocytosis; negative regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; membrane hyperpolarization; positive regulation of cellular protein metabolic process; response to glucocorticoid stimulus; negative regulation of smooth muscle cell migration; glucose homeostasis; negative regulation of granulocyte differentiation; positive regulation of interleukin-8 production; positive regulation of glucose import; negative regulation of gluconeogenesis; response to glucose stimulus; adiponectin-mediated signaling pathway; negative regulation of protein amino acid autophosphorylation; negative regulation of blood pressure; negative regulation of cell migration; response to nutrient; protein homooligomerization; generation of precursor metabolites and energy; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of heterotypic cell-cell adhesion; positive regulation of signal transduction; glucose metabolic process; negative regulation of tumor necrosis factor production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of fat cell differentiation; negative regulation of synaptic transmission; response to sucrose stimulus; membrane depolarization; positive regulation of peptidyl-tyrosine phosphorylation; fatty acid beta-oxidation; positive regulation of fatty acid metabolic process; response to ethanol; negative regulation of macrophage differentiation; negative regulation of low-density lipoprotein receptor biosynthetic process; cellular response to insulin stimulus; negative regulation of inflammatory response; response to hypoxia; brown fat cell differentiation; fatty acid oxidation; positive regulation of protein amino acid phosphorylation; response to activity; negative regulation of transcription, DNA-dependent; positive regulation of myeloid cell apoptosis; positive regulation of blood pressure

Disease: Adiponectin, Serum Level Of, Quantitative Trait Locus 1