Rabbit anti-Human CDKL1 Polyclonal Antibody | anti-CDKL1 antibody

CDKL1 Rabbit pAb

Cat. Num. AAA4758682

• Gene Names: CDKL1; P42; KKIALRE
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: CDKL1; Polyclonal Antibody; CDKL1 Rabbit pAb; P42; KKIALRE; anti-CDKL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-CDKL1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000
• Immunogen: Synthetic peptide of human CDKL1
• Conjugation: Unconjugated
• Molecular Function: The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated by the MAP kinases ERK 1 and ERK 2. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. The protein kinase p56 KKIAMRE is distantly related to the MAP kinase group of proteins and is closely related to p42 KKIALRE. KKIAMRE is predominantly expressed in testis, kidney, brain and lung. KKIAMRE contains the conserved MAP kinase dual phosphorylation motif in the sequence Thr-Asp-Tyr and is activated by treatment of cells by EGF. However, unlike other MAP kinases, the EGF-stimulated kinase activity does not require phosphorylation of KKIAMRE and KKIALRE in the Thr-Asp-Tyr motif.
• Modification: Unmodified
• Cell Position: Cytoplasm, Nucleus
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human prostate tissue and Human stomach tissue lysates, Primary antibody: 863416(CDKL1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 863416(CDKL1 Antibody) at dilution 1/20. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 863416(CDKL1 Antibody) at dilution 1/20. (Original magnification: ×200))

Product Categories/Family for anti-CDKL1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 37596297
• NCBI GeneID: 8814
• NCBI Accession #: NP_004187
• NCBI GenBank Nucleotide #: NM_004196.4
• UniProt Accession #: Q00532; Q2M3A4; Q6QUA0; Q8WXQ5
• Molecular Weight: 42 kDa
• NCBI Official Full Name: cyclin-dependent kinase-like 1 isoform 1
• NCBI Official Synonym Full Names: cyclin dependent kinase like 1
• NCBI Official Symbol: CDKL1
• NCBI Official Synonym Symbols: P42; KKIALRE
• NCBI Protein Information: cyclin-dependent kinase-like 1
• UniProt Protein Name: Cyclin-dependent kinase-like 1
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDKL1
• UniProt Entry Name: CDKL1_HUMAN

NCBI Description

This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

CDKL1: a protein kinase of the CDKL family. Accumulates primarily in the nucleus.

Protein type: Kinase, protein; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Cell cycle regulation; CMGC group; CDKL family

Chromosomal Location of Human Ortholog: 14q21.3

Cellular Component: cytoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase activity; ATP binding

Biological Process: regulation of cell cycle; heart development; protein amino acid phosphorylation