Prefoldin Subunit 1 Recombinant Protein | PFDN1 recombinant protein

Recombinant Prefoldin Subunit 1 (PFDN1)

Cat. Num. AAA2030112

• Gene Names: PFDN1; PDF; PFD1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Prefoldin Subunit 1; Recombinant Prefoldin Subunit 1 (PFDN1); PFDN1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MAAPVDLELK KAFTELQAKV IDTQQKVKLA DIQIEQLNRT KKHAHLTDTEIMTLVDETNM YEGVGRMFIL QSKEAIHSQL LEKQKIAEEK IKELEQKKSYLERSVKEAED NIREMLMARR AQ
• Sequence Length: 122

• Applicable Applications for PFDN1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 17.9kDa
• Accurate Molecular Mass (KD): 18kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Gln122
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21536449
• NCBI GeneID: 5201
• NCBI Accession #: NP_002613.2
• NCBI GenBank Nucleotide #: NM_002622.4
• Molecular Weight: 14,210 Da
• NCBI Official Full Name: prefoldin subunit 1
• NCBI Official Synonym Full Names: prefoldin subunit 1
• NCBI Official Symbol: PFDN1
• NCBI Official Synonym Symbols: PDF; PFD1
• NCBI Protein Information: prefoldin subunit 1
• UniProt Protein Name: Prefoldin subunit 1
• Protein Family: Prefoldin
• UniProt Gene Name: PFDN1
• UniProt Synonym Gene Names: PFD1
• UniProt Entry Name: PFD1_HUMAN

NCBI Description

This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. [provided by RefSeq, Jul 2008]

Uniprot Description

PFDN1: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Belongs to the prefoldin subunit beta family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: prefoldin complex

Molecular Function: unfolded protein binding; transcription factor activity

Biological Process: 'de novo' posttranslational protein folding; telencephalon development; cellular protein metabolic process; protein folding; B cell activation; regulation of transcription, DNA-dependent; cerebellum development; actin cytoskeleton organization and biogenesis; cell cycle