Western Blot (WB)
(WB Suggested Anti-CDK8 Antibody Titration: 1.25ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate)
Rabbit CDK8 Polyclonal Antibody | anti-CDK8 antibody
CDK8 antibody - C-terminal region
Western Blot (WB)
(WB Suggested Anti-CDK8 Antibody Titration: 1.25ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate)
Western Blot (WB)
(WB Suggested Anti-CDK8 Antibody Titration: 1.25ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate)
Target Description: The protein encoded by CDK8 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]
Uniprot Description
CDK8: a protein kinase of the CDK family. Along with cyclin C forms part of the RNA polymerase II holoenzyme complex. Phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. Regulates transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.
Protein type: Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; EC 2.7.11.23; EC 2.7.11.22; Kinase, protein; Protein kinase, CMGC; CMGC group; CDK family; CDK8 subfamily
Chromosomal Location of Human Ortholog: 13q12
Cellular Component: nucleoplasm; Srb-mediator complex; nucleus
Molecular Function: RNA polymerase subunit kinase activity; protein binding; cyclin-dependent protein kinase activity; ATP binding; protein kinase activity
Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; regulation of cell cycle; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; protein amino acid phosphorylation
Research Articles on CDK8
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Product Notes
The CDK8 cdk8 (Catalog #AAA3224396) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDK8 antibody - C-terminal region reacts with Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's CDK8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CDK8 cdk8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: GLIMTSDYQR SNPHAAYPNP GPSTSQPQSS MGYSATSQQP PQYSHQTHRY. It is sometimes possible for the material contained within the vial of "CDK8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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