Rabbit anti-Human Cdk6 Polyclonal Antibody | anti-Cdk6 antibody

Cdk6, CT (Cdk6, Cdkn6, Crk2, Cyclin-dependent kinase 6, CR2 protein kinase, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE) (Azide free) (HRP)

Cat. Num. AAA6284461

• Gene Names: Cdk6; Crk2; AI504062; 5830411I20
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Cdk6; Polyclonal Antibody; CT (Cdk6; Cdkn6; Crk2; Cyclin-dependent kinase 6; CR2 protein kinase; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE) (Azide free) (HRP); Serine/threonine-protein kinase PLSTIRE; anti-Cdk6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Cdk6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Cdk6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-312 amino acids from the C-terminal region of mouse Cdk6.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Cdk6 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Cdk6 antibody detected the Cdk6 protein (arrow).)

Related Product Information for anti-Cdk6 antibody

Cdk6 probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins (By similarity).

Product Categories/Family for anti-Cdk6 antibody

Antibodies; Protein Kinases

References

Puyol, M., et al. Cancer Cell 18(1):63-73(2010). Wiedemeyer, W.R., et al. Proc. Natl. Acad. Sci. U.S.A. 107(25):11501-11506(2010). Rivadeneira, D.B., et al. Gastroenterology 138(5):1920-1930(2010). Michaud, K., et al. Cancer Res. 70(8):3228-3238(2010). Choe, K.S., et al. J. Biol. Chem. 285(5):3044-3052(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 14149637
• NCBI GeneID: 12571
• NCBI Accession #: NP_034003.1
• NCBI GenBank Nucleotide #: NM_009873.2
• UniProt Accession #: Q64261; Q9R1D2; Q9R1D3
• Molecular Weight: 37,028 Da
• NCBI Official Full Name: cyclin-dependent kinase 6
• NCBI Official Synonym Full Names: cyclin-dependent kinase 6
• NCBI Official Symbol: Cdk6
• NCBI Official Synonym Symbols: Crk2; AI504062; 5830411I20
• NCBI Protein Information: cyclin-dependent kinase 6; CR2 protein kinase; cell division protein kinase 6; serine/threonine-protein kinase PLSTIRE
• UniProt Protein Name: Cyclin-dependent kinase 6
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk6
• UniProt Synonym Gene Names: Cdkn6; Crk2; CRK2
• UniProt Entry Name: CDK6_MOUSE

Uniprot Description

CDK6: a protein kinase of the CDK family. Important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. Phosphorylates and regulates the activity of the Rb tumor suppressor protein. Overexpressed and/or disrupted by translocation in leukemias, lymphomas and other cancers and amplified in gliomas and rodent cancers.

Protein type: Kinase, protein; Protein kinase, CMGC; Cell cycle regulation; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily

Cellular Component: nucleoplasm; ruffle; centrosome; cell projection; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; cyclin binding; cyclin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: Notch signaling pathway; negative regulation of myeloid cell differentiation; gliogenesis; T cell differentiation in the thymus; cell cycle; protein amino acid phosphorylation; regulation of cell proliferation; negative regulation of cell cycle; regulation of erythrocyte differentiation; positive regulation of fibroblast proliferation; regulation of gene expression; cell division; cell dedifferentiation; hemopoietic progenitor cell differentiation; hemopoiesis; negative regulation of osteoblast differentiation; positive regulation of cell-matrix adhesion; cell differentiation; phosphorylation; negative regulation of epithelial cell proliferation