Western Blot (WB)
(Western blot analysis of CDK5RAP1 Antibody (C-term) in K562 cell line lysates (35ug/lane). CDK5RAP1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human CDK5RAP1 Polyclonal Antibody | anti-CDK5RAP1 antibody
CDK5RAP1, CT (CDK5RAP1, C20orf34, CDK5 regulatory subunit-associated protein 1, CDK5 activator-binding protein C42)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western blot analysis of CDK5RAP1 Antibody (C-term) in K562 cell line lysates (35ug/lane). CDK5RAP1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CDK5RAP1 Antibody (C-term) in K562 cell line lysates (35ug/lane). CDK5RAP1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5RAP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5RAP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CDK5RAP1 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CDK5RAP1 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a regulator of cyclin-dependent kinase 5 activity. This protein has also been reported to modify RNA by adding a methylthio-group and may thus have a dual function as an RNA methylthiotransferase and as an inhibitor of cyclin-dependent kinase 5 activity. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013]
Uniprot Description
CDK5RAP1: Specifically inhibits CDK5 activation by CDK5R1. Belongs to the methylthiotransferase family. MiaB subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 20q11.21
Cellular Component: cytoplasm
Molecular Function: transferase activity; metal ion binding; 4 iron, 4 sulfur cluster binding; protein complex binding; protein kinase binding
Biological Process: regulation of neuron differentiation; negative regulation of cyclin-dependent protein kinase activity; brain development; tRNA modification
Research Articles on CDK5RAP1
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Product Notes
The CDK5RAP1 cdk5rap1 (Catalog #AAA6005042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDK5RAP1, CT (CDK5RAP1, C20orf34, CDK5 regulatory subunit-associated protein 1, CDK5 activator-binding protein C42) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDK5RAP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the CDK5RAP1 cdk5rap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK5RAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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