Rabbit anti-Human CDK3 Polyclonal Antibody | anti-CDK3 antibody

CDK3, NT (Y19) (CDK3, CDKN3, Cyclin-dependent kinase 3, Cell division protein kinase 3) (MaxLight 550)

Cat. Num. AAA6284387

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CDK3; Polyclonal Antibody; NT (Y19) (CDK3; CDKN3; Cyclin-dependent kinase 3; Cell division protein kinase 3) (MaxLight 550); Cell division protein kinase 3; anti-CDK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CDK3
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-CDK3 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CDK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14~43 amino acids from the N-terminal region of human CDK3.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDK3(arrow) using rabbit polyclonal CDK3 Antibody (N-term Y19). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK3 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CDK3 Antibody (N-term Y19), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using CDK3 Antibody (N-term Y19)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CDK3 antibody

This gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0.

Product Categories/Family for anti-CDK3 antibody

Antibodies; Protein Kinases

References

Bullrich, F., et al., Cancer Res. 55(6):1199-1205 (1995). Meyerson, M., et al., EMBO J. 11(8):2909-2917 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4557439
• NCBI GeneID: 1018
• NCBI Accession #: NP_001249.1
• NCBI GenBank Nucleotide #: NM_001258.2
• UniProt Accession #: Q00526
• Molecular Weight: 35,046 Da
• NCBI Official Full Name: cyclin-dependent kinase 3
• NCBI Official Synonym Full Names: cyclin-dependent kinase 3
• NCBI Official Symbol: CDK3
• NCBI Protein Information: cyclin-dependent kinase 3; cell division protein kinase 3
• UniProt Protein Name: Cyclin-dependent kinase 3
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK3
• UniProt Synonym Gene Names: CDKN3
• UniProt Entry Name: CDK3_HUMAN

NCBI Description

This gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK3: a protein kinase of the CDK family. May be involved in cell cycle control. Can phosphorylate histone H1.

Protein type: Protein kinase, CMGC; Cell cycle regulation; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily

Chromosomal Location of Human Ortholog: 17q25.1

Molecular Function: cyclin-dependent protein kinase activity; ATP binding

Biological Process: mitosis; cell proliferation; G0 to G1 transition; regulation of cell cycle; cell division; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle