Rabbit CDC7 Polyclonal Antibody | anti-CDC7 antibody

Rabbit Polyclonal to Human CDC7

Cat. Num. AAA247496

• Gene Names: CDC7; Hsk1; CDC7L1; HsCDC7; huCDC7
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: CDC7; Polyclonal Antibody; Rabbit Polyclonal to Human CDC7; Anti-CDC7 Antibody (Kinase Domain) IHC-plus; Cdc7 Kinase; CDC7-like 1; CDC7L1; HsCDC7; HuCDC7; CDC7-related kinase; Hsk1; MuCdc7; Human CDC7; anti-CDC7 antibody

• Host: Rabbit
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CDC7. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 574

• Applicable Applications for anti-CDC7 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (5.2 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 19 amino acid peptide from kinase domain of human CDC7. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon (95%).
• Immunogen Type: Synthetic peptide
• Immunogen: CDC7 antibody was raised against synthetic 19 amino acid peptide from kinase domain of human CDC7. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon (95%).
• Antigen Modification: Kinase Domain
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-CDC7 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-CDC7 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-CDC7 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-CDC7 antibody

CDC7L1, CDC7 type protein kinase, regulates firing of the origins of initiation in DNA replication. CDC7L1 forms a complex with a Dbf4-related regulatory subunit to generate an active kinase. Mini-chromosome maintenance protein (MCM) is the major physiological target of Cdc7L1. At least three mRNA variants have been reported. Overexpression of the CDC7L1 protein may be associated with neoplastic transformation for some tumors.

Product Categories/Family for anti-CDC7 antibody

Family: Protein Kinase; Subfamily: CDC7

NCBI and Uniprot Product Information

• NCBI GI #: 4502715
• NCBI GeneID: 8317
• NCBI Accession #: NP_003494.1
• NCBI GenBank Nucleotide #: NM_003503.3
• UniProt Accession #: O00311; O00558; Q5T5U5; D3DT31
• Molecular Weight: 63,888 Da
• NCBI Official Full Name: cell division cycle 7-related protein kinase
• NCBI Official Synonym Full Names: cell division cycle 7
• NCBI Official Symbol: CDC7
• NCBI Official Synonym Symbols: Hsk1; CDC7L1; HsCDC7; huCDC7
• NCBI Protein Information: cell division cycle 7-related protein kinase; CDC7-related kinase; cell division cycle 7 homolog; cell division cycle 7-like protein 1; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1
• UniProt Protein Name: Cell division cycle 7-related protein kinase
• Protein Family: Cell division control protein
• UniProt Gene Name: CDC7
• UniProt Synonym Gene Names: CDC7L1; CDC7-related kinase; HsCdc7; huCdc7
• UniProt Entry Name: CDC7_HUMAN

NCBI Description

This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008]

Uniprot Description

CDC7: an evolutionarily conserved serine/threonine protein kinase of the CDC7 family. Predominantly localized in the nucleus, it plays a pivotal role in linking cell cycle regulation to genome duplication, being essential for the firing of DNA replication origins. Plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. The human protein requires phosphorylation by CDK1 at T376 and binding to activator of S-phase kinase (ASK)/DBF4B to be fully activated. The interaction between Cdc7 and DBFK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. Although CDC7 expression levels appear to be constant throughout the cell cycle, the expression levels of DBF4B is cell cycle-regulated. Thus, the CDC7 activity is activated during S phase in eukaryotes. The Cdc7/ DBF4B complex regulates initiation of DNA replication by phosphorylating chromatin-bound mini-chromosome maintenance protein 2 (MCM2). Impaired Cdc7 kinase activity leads to S-phase arrest. Plays a role in the TGF-beta regulation of smooth muscle cell (SMC) differentiation. Interacts with Smad3 to facilitate Smad3 binding to SMC marker promoter, leading to the activation of SMC marker gene transcription.

Protein type: Protein kinase, Other; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; CDC7 family

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: nucleoplasm; microtubule cytoskeleton; intercellular bridge; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of cell shape; peptidyl-serine phosphorylation; DNA replication initiation; cell division; positive regulation of cell proliferation; mitotic cell cycle; DNA replication; phagocytosis; G1/S transition of mitotic cell cycle; double-strand break repair via break-induced replication