Western Blot (WB)
(The anti-Phospho-CDC6-S54 Pab is used in Western blot to detect Phospho-CDC6-S54 in Ramos tissue lysate)
Rabbit anti-Human CDC6 Ser54 Polyclonal Antibody
CDC6, phosphorylated Ser54 (Cell Division Control Protein 6 Homolog, CDC6-related Protein, p62(cdc6), HsCDC6, Cdc18-related Protein, HsCDC18, CDC18L)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
CDC6 Ser54; Polyclonal Antibody; CDC6; phosphorylated Ser54 (Cell Division Control Protein 6 Homolog; CDC6-related Protein; p62(cdc6); HsCDC6; Cdc18-related Protein; HsCDC18; CDC18L); Anti -CDC6; anti-CDC6 Ser54 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Cdc6 phosphorylated at Ser55.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-CDC6 Ser54 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser54 of human Cdc6 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(The anti-Phospho-CDC6-S54 Pab is used in Western blot to detect Phospho-CDC6-S54 in Ramos tissue lysate)
Western Blot (WB)
(The anti-Phospho-CDC6-S54 Pab is used in Western blot to detect Phospho-CDC6-S54 in Ramos tissue lysate)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-CDC6 Ser54 antibody
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
Product Categories/Family for anti-CDC6 Ser54 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Cdc6
Similar Products
Product Notes
The CDC6 Ser54 (Catalog #AAA626932) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC6, phosphorylated Ser54 (Cell Division Control Protein 6 Homolog, CDC6-related Protein, p62(cdc6), HsCDC6, Cdc18-related Protein, HsCDC18, CDC18L) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDC6 Ser54 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the CDC6 Ser54 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC6 Ser54, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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