Goat CDC48/ VCP/YDL126C Polyclonal Antibody | anti-CDC48 antibody

Goat anti-CDC48/ VCP/YDL126C (baker's yeast, aa128-142) Antibody

Cat. Num. AAA423252

• Reactivity: Human; Expected from sequence similarity: Saccharomyces cerevisiae S288c, Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CDC48/ VCP/YDL126C; Polyclonal Antibody; Goat anti-CDC48/ VCP/YDL126C (baker's yeast; aa128-142) Antibody; CDC48; CDC48p; YDL126C; VCP; transitional endoplasmic reticulum ATPase; valosin containing protein; p97; TERA; CDC48 antibody; CDC48p antibody; YDL126C antibody; VCP antibody; transitional endoplasmic reticulum ATPase antibody; valosin containing protein antibody; p97 antibody; TERA antibody; CDC48/ VCP / YDL126C (baker's yeast; aa128-142); anti-CDC48 antibody

• Host: Goat
• Reactivity: Human; Expected from sequence similarity: Saccharomyces cerevisiae S288c, Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: PIADTIEGITGNLFD
• Sequence Length: 835

• Applicable Applications for anti-CDC48 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 100kDa band observed in lysates of cell line HeLa (calculated MW of 92. 0kDa according to NP_010157.1). Recommended concentration: 0.3-1ug/ml.; Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
• Immunogen: Peptide with sequence C-PIADTIEGITGNLFD, from the internal region of the protein sequence according to NP_010157.1.
• Epitope: Internal region
• Note: This antibody may cross react in many other species.
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

(MBS423252 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining)

NCBI and Uniprot Product Information

• NCBI GI #: 6320077
• NCBI GeneID: 851431
• NCBI Accession #: NP_010157.1
• NCBI GenBank Nucleotide #: NM_001180185.1
• NCBI Official Full Name: AAA family ATPase CDC48
• NCBI Official Symbol: CDC48
• NCBI Protein Information: AAA family ATPase CDC48
• UniProt Protein Name: Cell division control protein 48
• Protein Family: Cell division cycle protein
• UniProt Gene Name: CDC48
• UniProt Entry Name: CDC48_YEAST

Uniprot Description

Involved in spindle disassembly, degradation of ubiquitinated proteins and protein export from the endoplasmic reticulum to the cytoplasm. Acts as a chaperone that collects ubiquitinated substrates. Has a role in the endoplasmic reticulum-associated degradation (ERAD) pathway. Required for the proteasome-dependent processing/activation of MGA2 and SPT23 transcription factors leading to the subsequent expression of OLE1. Has an additional role in the turnover of OLE1 where it targets ubiquitinated OLE1 and other proteins to the ERAD (PubMed:11733065, PubMed:11740563, PubMed:11813000, PubMed:11847109, PubMed:21070972, PubMed:21148305). Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. CDC48 may provide the mechanical force that dislodges the polyubiquitinated nascent peptides from the exit channel (PubMed:23178123, PubMed:24261871).