Human Beta-Site APP-Cleaving Enzyme 1 (BACE1) ELISA Kit | BACE1 elisa kit

Human beta-Site APP-Cleaving Enzyme 1 (BACE1) ELISA Kit

Cat. Num. AAA3802298

• Gene Names: BACE1; ASP2; BACE; HSPC104
• Reactivity: Human
• Synonyms: Beta-Site APP-Cleaving Enzyme 1 (BACE1); Human beta-Site APP-Cleaving Enzyme 1 (BACE1) ELISA Kit; BACE1 elisa kit

• Reactivity: Human
• Sequence Length: 401

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 7.5 U/L-120 U/L
• Sensitivity: 0.1 U/L

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BACE1 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofBACE1 in the sample, This BACE1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusBACE1 concentration. The concentration ofBACE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 333440466
• NCBI GeneID: 23621
• NCBI Accession #: NP_001193977.1
• NCBI GenBank Nucleotide #: NP_001193977.1
• UniProt Accession #: P56817
• NCBI Official Full Name: beta-secretase 1 isoform E
• NCBI Official Synonym Full Names: beta-secretase 1
• NCBI Official Symbol: BACE1
• NCBI Official Synonym Symbols: ASP2; BACE; HSPC104
• NCBI Protein Information: beta-secretase 1
• UniProt Protein Name: Beta-secretase 1
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE1
• UniProt Synonym Gene Names: BACE; KIAA1149; ASP2; Asp 2
• UniProt Entry Name: BACE1_HUMAN

NCBI Description

This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015]

Uniprot Description

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.

Protein type: Protease; EC 3.4.23.46; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.2-q23.3

Cellular Component: multivesicular body; Golgi apparatus; cell surface; cytoplasmic vesicle membrane; integral to plasma membrane; axon; endoplasmic reticulum; late endosome; integral to membrane; plasma membrane; trans-Golgi network; endosome

Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity

Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis