Monkey Substance P ELISA Kit | S.P elisa kit

Monkey Substance P ELISA Kit

Cat. Num. AAA738011

• Gene Names: Tac1; TAC; PPTA3; Ppt5fl; RATPPTA3
• Reactivity: Monkey
• Synonyms: Substance P; Monkey Substance P ELISA Kit; S.P elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This SP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey SP. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This SP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey SP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S.P elisa kit

Principle of the Assay: SP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SP antibody and an SP-HRP conjugate. The assay sample and buffer are incubated together with SP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SP concentration since SP from samples and SP-HRP conjugate compete for the anti-SP antibody binding site. Since the number of sites is limited, as more sites are occupied by SP from the sample, fewer sites are left to bind SP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for S.P elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 56068
• NCBI GeneID: 24806
• UniProt Accession #: P06767; P08856; P08857; P22356; Q6LD93
• Molecular Weight: 11,374 Da
• NCBI Official Full Name: substance P
• NCBI Official Synonym Full Names: tachykinin, precursor 1
• NCBI Official Symbol: Tac1
• NCBI Official Synonym Symbols: TAC; PPTA3; Ppt5fl; RATPPTA3
• NCBI Protein Information: protachykinin-1; PPT; tachykinin 1; Tachykinin (substance P, neurokinin A, neuropeptide K, neuropeptide gamma)
• UniProt Protein Name: Protachykinin-1
• Protein Family: Substance
• UniProt Gene Name: Tac1
• UniProt Synonym Gene Names: Nka; Nkna; Tac2; NKA; NPK
• UniProt Entry Name: TKN1_RAT

NCBI Description

encodes neuropeptides substance P, neurokinin A, neuropeptide K, and neuropeptide gamma [RGD, Feb 2006]

Uniprot Description

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.