Rabbit anti-Human CDC42EP3 Polyclonal Antibody | anti-CDC42EP3 antibody

CDC42EP3, NT (CDC42EP3, BORG2, CEP3, Cdc42 effector protein 3, Binder of Rho GTPases 2, MSE55-related Cdc42-binding protein) (AP)

Cat. Num. AAA6283940

• Gene Names: CDC42EP3; UB1; CEP3; BORG2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CDC42EP3; Polyclonal Antibody; NT (CDC42EP3; BORG2; CEP3; Cdc42 effector protein 3; Binder of Rho GTPases 2; MSE55-related Cdc42-binding protein) (AP); MSE55-related Cdc42-binding protein; anti-CDC42EP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CDC42EP3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CDC42EP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~32 amino acids from the N-term region of human CDC42EP3.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CDC42EP3 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDC42EP3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with CDC42EP3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of CDC42EP3 (arrow) using rabbit polyclonal CDC42EP3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC42EP3 gene.)

Flow Cytometry (FC/FACS)

(CDC42EP3 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CDC42EP3 antibody

CDC42EP3 is probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. It induces pseudopodia formation in fibroblasts.

Product Categories/Family for anti-CDC42EP3 antibody

Antibodies; Cell Division Cycle (CDC)

References

Simpson, J.C., et.al., EMBO Rep. 1 (3), 287-292 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 19923356
• NCBI GeneID: 10602
• NCBI Accession #: NP_006440
• NCBI GenBank Nucleotide #: NM_006449.4
• UniProt Accession #: Q9UKI2; O95353; Q9UQJ0; B2R8S0
• Molecular Weight: 27,678 Da
• NCBI Official Full Name: cdc42 effector protein 3
• NCBI Official Synonym Full Names: CDC42 effector protein 3
• NCBI Official Symbol: CDC42EP3
• NCBI Official Synonym Symbols: UB1; CEP3; BORG2
• NCBI Protein Information: cdc42 effector protein 3
• UniProt Protein Name: Cdc42 effector protein 3
• Protein Family: Cdc42 effector protein
• UniProt Gene Name: CDC42EP3
• UniProt Synonym Gene Names: BORG2; CEP3
• UniProt Entry Name: BORG2_HUMAN

NCBI Description

This gene encodes a member of a small family of guanosine triphosphate (GTP) metabolizing proteins that contain a CRIB (Cdc42, Rac interactive binding) domain. Members of this family of proteins act as effectors of CDC42 function. The encoded protein is involved in actin cytoskeleton re-organization during cell shape changes, including pseudopodia formation. A pseudogene of this gene is found on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

Uniprot Description

CDC42EP3: Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. Belongs to the BORG/CEP family.

Protein type: G protein regulator, misc.

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: cytoplasm; plasma membrane; endomembrane system; actin cytoskeleton

Molecular Function: GTP-Rho binding; cytoskeletal regulatory protein binding

Biological Process: regulation of cell shape; positive regulation of pseudopodium formation; positive regulation of actin filament polymerization; signal transduction; Rho protein signal transduction