Western Blot (WB)
(Western blot analysis of CDC42EP3 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDC42EP3 (arrow) was detected using the purified Pab.)
Rabbit anti-Human CDC42EP3 Polyclonal Antibody | anti-CDC42EP3 antibody
CDC42EP3, NT (CDC42EP3, BORG2, CEP3, Cdc42 effector protein 3, Binder of Rho GTPases 2, MSE55-related Cdc42-binding protein) (APC)
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of CDC42EP3 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDC42EP3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CDC42EP3 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDC42EP3 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with CDC42EP3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with CDC42EP3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(Western blot analysis of CDC42EP3 (arrow) using rabbit polyclonal CDC42EP3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC42EP3 gene.)
Western Blot (WB)
(Western blot analysis of CDC42EP3 (arrow) using rabbit polyclonal CDC42EP3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDC42EP3 gene.)
Flow Cytometry (FC/FACS)
(CDC42EP3 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CDC42EP3 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a small family of guanosine triphosphate (GTP) metabolizing proteins that contain a CRIB (Cdc42, Rac interactive binding) domain. Members of this family of proteins act as effectors of CDC42 function. The encoded protein is involved in actin cytoskeleton re-organization during cell shape changes, including pseudopodia formation. A pseudogene of this gene is found on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
Uniprot Description
CDC42EP3: Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. Belongs to the BORG/CEP family.
Protein type: G protein regulator, misc.
Chromosomal Location of Human Ortholog: 2p21
Cellular Component: cytoplasm; plasma membrane; endomembrane system; actin cytoskeleton
Molecular Function: GTP-Rho binding; cytoskeletal regulatory protein binding
Biological Process: regulation of cell shape; positive regulation of pseudopodium formation; positive regulation of actin filament polymerization; signal transduction; Rho protein signal transduction
Research Articles on CDC42EP3
Similar Products
Product Notes
The CDC42EP3 cdc42ep3 (Catalog #AAA6283941) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC42EP3, NT (CDC42EP3, BORG2, CEP3, Cdc42 effector protein 3, Binder of Rho GTPases 2, MSE55-related Cdc42-binding protein) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDC42EP3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CDC42EP3 cdc42ep3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC42EP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
