anti-Human Cdc37 Polyclonal Antibody | anti-CDC37 antibody

Anti-Cdc37 Antibody

Cat. Num. AAA178381

• Gene Names: CDC37; P50CDC37
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: Cdc37; Polyclonal Antibody; Anti-Cdc37 Antibody; Hsp90 co-chaperone Cdc37; CDC 37; CDC37; CDC37 cell division cycle 37 homolog; CDC37 cell division cycle 37 S cerevisiae homolog; Cdc37 homolog; CDC37 protein; CDC37_HUMAN; CDC37A; Cell division cycle 37 homolog; Hsp90 chaperone protein kinase targeting subunit; Hsp90 chaperone protein kinase targeting subunit p50Cdc37; Hsp90 chaperone protein kinase-targeting subunit; Hsp90 co chaperone Cdc37; p50; p50Cdc37; S cerevisiae hypothetical protein CDC37; S. cerevisiae hypothetical protein CDC37 antibody; cell division cycle 37; anti-CDC37 antibody

• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 378

• Applicable Applications for anti-CDC37 antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml
• Immunogen: E Coli-derived human Cdc37 recombinant protein (Position: M216-V378). Human Cdc37 shares 95.7% and 95.1% amino acid (aa) sequence identity with mouse and rat Cdc37, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Cdc37 Picoband antibody, MBS178381, Western blottingAll lanes: Anti Cdc37 (MBS178381) at 0.5ug/mlWB: 293T Whole Cell Lysate at 40ugPredicted bind size: 44KDObserved bind size: 44KD )

Related Product Information for anti-CDC37 antibody

Description: Rabbit IgG polyclonal antibody for Hsp90 co-chaperone Cdc37(CDC37) detection. Tested with WB in Human.

Background: Hsp90 co-chaperone Cdc37, also called P50CDC37, is a protein that in humans is encoded by the CDC37 gene. The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This gene is mapped to 19p13.2. And this protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.

References

1. Chen, G., Cao, P., Goeddel, D. V. TNF-induced recruitment and activation of the IKK complex require Cdc37 and Hsp90. Molec. Cell 9: 401-410, 2002. 2. Vaughan, C. K., Mollapour, M., Smith, J. R., Truman, A., Hu, B., Good, V. M., Panaretou, B., Neckers, L., Clarke, P. A., Workman, P., Piper, P. W., Prodromou, C., Pearl, L. H.Hsp90-dependent activation of protein kinases is regulated by chaperone-targeted dephosphorylation of Cdc37. Molec. Cell 31: 886-895, 2008.

NCBI and Uniprot Product Information

• NCBI GI #: 5901922
• NCBI GeneID: 11140
• NCBI Accession #: NP_008996.1
• NCBI GenBank Nucleotide #: NM_007065.3
• UniProt Accession #: Q16543; Q53YA2
• Molecular Weight: 44,468 Da
• NCBI Official Full Name: hsp90 co-chaperone Cdc37
• NCBI Official Synonym Full Names: cell division cycle 37
• NCBI Official Symbol: CDC37
• NCBI Official Synonym Symbols: P50CDC37
• NCBI Protein Information: hsp90 co-chaperone Cdc37
• UniProt Protein Name: Hsp90 co-chaperone Cdc37
• Protein Family: Hsp90 co-chaperone
• UniProt Gene Name: CDC37
• UniProt Synonym Gene Names: CDC37A
• UniProt Entry Name: CDC37_HUMAN

NCBI Description

The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC37: a molecular chaperone that functions in cell signal transduction. Forms complexes with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. Also interacts with androgen receptor. It is thought to play a critical role in directing Hsp90 to its target kinases.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; cytosol

Molecular Function: chaperone binding; heat shock protein binding; Hsp90 protein binding; kinase binding; protein binding; protein kinase binding; protein kinase regulator activity; protein-tyrosine kinase activity; unfolded protein binding

Biological Process: peptidyl-tyrosine phosphorylation; protein folding; protein stabilization; protein targeting; regulation of cyclin-dependent protein kinase activity