Western Blot (WB)
(Western Blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: Jurkat)
Rabbit Cdc34 Polyclonal Antibody | anti-Cdc34 antibody
Cdc34 Polyclonal Antibody
FC/FACS: 1:50-1:100
ICC: 1:100-1:500
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: Jurkat)
Western Blot (WB)
(Western Blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: Jurkat)
Immunocytochemistry (ICC)
(ICC staining Cdc34 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Cdc34 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008]
Uniprot Description
CDC34: a member of the ubiquitin-conjugating enzyme family. Is a part of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which is required for ubiquitin-mediated degradation of cell cycle G1 regulators. Required for the initiation of DNA replication.
Protein type: Ligase; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; DNA replication initiation; positive regulation of neuron apoptosis; protein modification process; protein ubiquitination; G1/S transition of mitotic cell cycle
Research Articles on Cdc34
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Product Notes
The Cdc34 cdc34 (Catalog #AAA3012447) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cdc34 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cdc34 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1,000-1:5,000 FC/FACS: 1:50-1:100 ICC: 1:100-1:500. Researchers should empirically determine the suitability of the Cdc34 cdc34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdc34, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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