Rabbit Cdc25C Polyclonal Antibody | anti-Cdc25C antibody

Cdc25C (Phospho-S216) Polyclonal Antibody

Cat. Num. AAA3004549

• Gene Names: CDC25C; CDC25; PPP1R60
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cdc25C; Polyclonal Antibody; Cdc25C (Phospho-S216) Polyclonal Antibody; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; CDC25C; anti-Cdc25C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-CDC25C (S216) polyclonal antibody detects endogenous levels of CDC25C protein only when phosphorylated at Ser216.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 473

• Applicable Applications for anti-Cdc25C antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human Cdc25C around the phosphorylation site of Serine 216.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-Cdc25C (S216) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-Cdc25C antibody

Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

NCBI and Uniprot Product Information

• NCBI GI #: 125625350
• NCBI GeneID: 995
• NCBI Accession #: NP_001781.2
• NCBI GenBank Nucleotide #: NP_001781.2
• UniProt Accession #: P30307
• Molecular Weight: ~ 53kDa
• NCBI Official Full Name: M-phase inducer phosphatase 3 isoform a
• NCBI Official Synonym Full Names: cell division cycle 25C
• NCBI Official Symbol: CDC25C
• NCBI Official Synonym Symbols: CDC25; PPP1R60
• NCBI Protein Information: M-phase inducer phosphatase 3
• UniProt Protein Name: M-phase inducer phosphatase 3
• UniProt Gene Name: CDC25C
• UniProt Entry Name: MPIP3_HUMAN

NCBI Description

This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]

Uniprot Description

CDC25C: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; nucleus; cytosol

Molecular Function: protein binding; WW domain binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: cell proliferation; mitosis; viral reproduction; regulation of cell cycle; cell division; regulation of mitosis; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; DNA replication; G2/M transition of mitotic cell cycle