Rabbit CDC25A Polyclonal Antibody | anti-CDC25A antibody

CDC25A (Phospho-S124) Polyclonal Antibody

Cat. Num. AAA3005150

• Gene Names: CDC25A; CDC25A2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CDC25A; Polyclonal Antibody; CDC25A (Phospho-S124) Polyclonal Antibody; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A; anti-CDC25A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-CDC25A (S124) polyclonal antibody detects endogenous levels of Cdc25A protein only when phosphorylated at Ser124.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 524

• Applicable Applications for anti-CDC25A antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human Cdc25A around the phosphorylation site of Serine 124.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-CDC25A (S124) polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:Mouse spleen tissue lysateLane3:Rat liver tissue lysate)

Related Product Information for anti-CDC25A antibody

Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

NCBI and Uniprot Product Information

• NCBI GI #: 42490758
• NCBI GeneID: 993
• NCBI Accession #: NP_001780.2
• NCBI GenBank Nucleotide #: NP_001780.2
• UniProt Accession #: P30304
• Molecular Weight: ~ 59, 70kDa
• NCBI Official Full Name: M-phase inducer phosphatase 1 isoform a
• NCBI Official Synonym Full Names: cell division cycle 25A
• NCBI Official Symbol: CDC25A
• NCBI Official Synonym Symbols: CDC25A2
• NCBI Protein Information: M-phase inducer phosphatase 1
• UniProt Protein Name: M-phase inducer phosphatase 1
• Protein Family: M-phase inducer phosphatase
• UniProt Gene Name: CDC25A
• UniProt Entry Name: MPIP1_HUMAN

NCBI Description

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: mitosis; cell proliferation; response to radiation; regulation of cell cycle; cell division; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; G2/M transition of mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle