SLC3A2 / CD98 recombinant protein

Recombinant Human SLC3A2 / CD98 Protein (His tag)

Cat. Num. AAA2545545

• Gene Names: SLC3A2; 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: SLC3A2 / CD98; Recombinant Human SLC3A2 / CD98 Protein (His tag); 4F2; 4F2HC; 4T2HC; CD98; CD98HC; MDU1; NACAE; SLC3A2 / CD98 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 631

• Application Notes: The recombinant human SLC3A2 consists of 445 amino acids and has a calculated molecular mass of 49.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSLC3A2 is approximately 65-75 kDa due to glycosylation.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SLC3A2 / CD98 recombinant protein

Background: 4F2 cell-surface antigen heavy chain, also known as 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, CD98, SLC3A2 and MDU1, is a single-pass type I I membrane protein which belongs to the SLC3A transporter family. SLC3A2 / MDU1 is expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta is significantly stronger at full-term than at the mid-trimester stage. SLC3A2 / MDU1 is expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. It is expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. SLC3A2 / MDU1 is also expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco2-BBE. SLC3A2 / MDU1 is required for the function of light chain amino-acid transporters. It involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. SLC3A2 / MDU1 is involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, SLC3A2 / MDU1 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. SLC3A2 / MDU1 plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. It is required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, SLC3A2 / MDU1 is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta.

Description: A DNA sequence encoding the human SLC3A2 isoform 1 (P08195-1) (Arg 206-Ala 630) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 61744477
• NCBI GeneID: 6520
• NCBI Accession #: NP_001012680.1
• NCBI GenBank Nucleotide #: NM_001012662.2
• UniProt Accession #: P08195; Q13543
• Molecular Weight: 71,123 Da
• NCBI Official Full Name: 4F2 cell-surface antigen heavy chain isoform b
• NCBI Official Synonym Full Names: solute carrier family 3 member 2
• NCBI Official Symbol: SLC3A2
• NCBI Official Synonym Symbols: 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
• NCBI Protein Information: 4F2 cell-surface antigen heavy chain
• UniProt Protein Name: 4F2 cell-surface antigen heavy chain
• UniProt Gene Name: SLC3A2
• UniProt Synonym Gene Names: MDU1; 4F2hc

NCBI Description

This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized. [provided by RefSeq, Nov 2010]

Uniprot Description

SLC3A2: a transmembrane solute carrier protein that transports L-type amino acids and regulates intracellular calcium levels. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. Disulfide-linked heterodimer of a glycosylated heavy chain and a non-glycosylated light chain (SLC7A5, SLC7A6, SLCA7A7, SLC7A8, SLC7A10 or SLCA7A11). Colocalizes with cadherins. Interacts with FAM57A and ICAM1. Constitutively and specifically associates with beta-1 integrins (alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1 and alpha-6/beta-1), but minimally with alpha-4/beta-1. Expression is induced in resting peripheral blood T- lymphocytes following PHA stimulation. Expression increases at the time of maximal DNA synthesi, in fibroblasts stimulated to divide. Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid- trimester stage. Belongs to the SLC3A transporter family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: cell surface; cell-cell adherens junction; cytoplasm; membrane; nucleus; plasma membrane

Molecular Function: calcium:sodium antiporter activity; double-stranded RNA binding; neutral amino acid transmembrane transporter activity; protein binding

Biological Process: amino acid transport; leukocyte migration; response to exogenous dsRNA; tryptophan transport