Western Blot (WB)
(Anti-Cd97 rabbit polyclonal antibody at 1:500 dilution Lane A: NIH/3T3 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Raw264.7 Whole Cell Lysate Lane D: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:92 kDa)
Rabbit anti-Mouse CD97 Polyclonal Antibody | anti-ADGRE5 antibody
Anti-CD97 Antibody, Rabbit Polyclonal
Gene Names
Cd97; TM7LN1; AA409984
Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
CD97; Polyclonal Antibody; Anti-CD97 Antibody; Rabbit Polyclonal; CD97/ADGRE5 Antibody; Rabbit PAb; Antigen Affinity Purified; adhesion G protein-coupled receptor E5; Anti-AA409984 Antibody; Anti-Cd97 Antibody; Anti-TM7LN1 Antibody; anti-ADGRE5 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse CD97/ADGRE5
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ADGRE5 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 5-10uL/mg of lysate
IP: 5-10uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the center region of the Mouse CD97/ADGRE5
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse CD97/ADGRE5, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-Cd97 rabbit polyclonal antibody at 1:500 dilution Lane A: NIH/3T3 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Raw264.7 Whole Cell Lysate Lane D: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:92 kDa)
Western Blot (WB)
(Anti-Cd97 rabbit polyclonal antibody at 1:500 dilution Lane A: NIH/3T3 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Raw264.7 Whole Cell Lysate Lane D: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:92 kDa)
Immunoprecipitation (IP)
(Cd97 was immunoprecipitated using: Lane A:0.5 mg NIH/3T3 Whole Cell Lysate 4 uL anti-Cd97 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Cd97 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa Observed band size :70 kDa)
Immunoprecipitation (IP)
(Cd97 was immunoprecipitated using: Lane A:0.5 mg NIH/3T3 Whole Cell Lysate 4 uL anti-Cd97 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Cd97 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa Observed band size :70 kDa)
Related Product Information for anti-ADGRE5 antibody
The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium-binding site. Mature CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adhere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isoforms that are related to the calcium-binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues.
References
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Gray JX, et al. (1996) CD97 is a processed, seven-transmembrane, heterodimeric receptor associated with inflammation. The journal of Immunology. 157 (12): 5438-47.Hamann J, et al. (1998) Characterization of the CD55 (DAF)-binding site on the seven-span transmembrane receptor CD97.European Journal of Immunology. 28 (5): 1701-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,413 Da
NCBI Official Full Name
CD97 antigen isoform 2
NCBI Official Synonym Full Names
CD97 antigen
NCBI Official Symbol
Cd97
NCBI Official Synonym Symbols
TM7LN1; AA409984
NCBI Protein Information
CD97 antigen; EGF-TM7 receptor
UniProt Protein Name
CD97 antigen
Protein Family
UniProt Gene Name
Cd97
UniProt Entry Name
CD97_MOUSE
Uniprot Description
CD97: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 2; Membrane protein, multi-pass
Cellular Component: focal adhesion; membrane; integral to membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcium ion binding
Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; cell adhesion; signal transduction
Research Articles on ADGRE5
Similar Products
Product Notes
The ADGRE5 cd97 (Catalog #AAA8101735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD97 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD97 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 5-10uL/mg of lysate. Researchers should empirically determine the suitability of the ADGRE5 cd97 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD97, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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