Immunofluorescence (IF)
(Immunofluorescence staining of Human CD97 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD97 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membranes.)
Mouse anti-Human CD97 Monoclonal Antibody | anti-ADGRE5 antibody
Anti-CD97 Antibody, Mouse Monoclonal
ICC/IF: 1:20-1:100
ELISA (Cap): 1:250-1:2000
ELISA (Cap): This antibody will detect Human CD97 in ELISA pair set.
In a sandwich ELISA, it can be used as capture antibody when paired with.
Immunofluorescence (IF)
(Immunofluorescence staining of Human CD97 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD97 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membranes.)
Immunofluorescence (IF)
(Immunofluorescence staining of Human CD97 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD97 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membranes.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]
Uniprot Description
CD97: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, GPCR; GPCR, family 2; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19p13.12
Cellular Component: extracellular space; focal adhesion; membrane; integral to plasma membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity; calcium ion binding
Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; cell-cell signaling; immune response; cell motility; inflammatory response; cell adhesion
Research Articles on ADGRE5
Similar Products
Product Notes
The ADGRE5 cd97 (Catalog #AAA8105214) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD97 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD97 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), ELISA (EIA) (Cap), Immunocytochemistry (ICC), Immunofluorescence (IF). ELISA: 1:1000-1:2000 ICC/IF: 1:20-1:100 ELISA (Cap): 1:250-1:2000 ELISA (Cap): This antibody will detect Human CD97 in ELISA pair set. In a sandwich ELISA, it can be used as capture antibody when paired with. Researchers should empirically determine the suitability of the ADGRE5 cd97 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD97, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
