Western Blot (WB)
(Figure 1. Western blot analysis of Orai2 using anti-Orai2 antibody (MBS1753764).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human A375 whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human HepG2 whole cell lysatesLane 4: human K562 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Orai2 antigen affinity purified polyclonal antibody (Catalog # MBS1753764) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Orai2 at approximately 39KD. The expected band size for Orai2 is at 39KD. )
Rabbit anti-Human Orai2 Polyclonal Antibody | anti-ORAI2 antibody
Anti-Orai2 Antibody
Gene Names
ORAI2; CBCIP2; C7orf19; MEM142B; TMEM142B
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Immunogen affinity purified.
Synonyms
Orai2; Polyclonal Antibody; Anti-Orai2 Antibody; ORAI2; C7orf19; CBCIP2; TMEM142B; PP1729; Protein orai-2; CAP-binding protein complex-interacting protein 2; Transmembrane protein 142B; ORAI calcium release-activated calcium modulator 2; anti-ORAI2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for Orai2 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-ORAI2 antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Immunogen
E Coli-derived human Orai2 recombinant protein (Position: H21-L254).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of Orai2 using anti-Orai2 antibody (MBS1753764).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human A375 whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human HepG2 whole cell lysatesLane 4: human K562 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Orai2 antigen affinity purified polyclonal antibody (Catalog # MBS1753764) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Orai2 at approximately 39KD. The expected band size for Orai2 is at 39KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Orai2 using anti-Orai2 antibody (MBS1753764).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human A375 whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human HepG2 whole cell lysatesLane 4: human K562 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Orai2 antigen affinity purified polyclonal antibody (Catalog # MBS1753764) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Orai2 at approximately 39KD. The expected band size for Orai2 is at 39KD. )
Related Product Information for anti-ORAI2 antibody
Protein orai-2 is a protein that in humans is encoded by the ORAI2 gene. Orai2 transcripts are predominantly expressed in kidney, lung, and spleen, and it appears that Orai2 is the only Orai isoform with multiple transcripts. Co-expresssion of Orai2 and STIM1 was shown to produce currents that appear similar but smaller than those produced by co-expression of Orai1 and STIM1.
References
1. Feske, S., Gwack, Y., Prakriya, M., Srikanth, S., Puppel, S. -H., Tanasa, B., Hogan, P. G., Lewis, R. S., Daly, M., Rao, A. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 441: 179-185, 2006.
2. Mercer, J. C., DeHaven, W. I., Smyth, J. T., Wedel, B., Boyles, R. R., Bird, G. S., Putney, J. W., Jr. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 281: 24979-24990, 2006.
2. Mercer, J. C., DeHaven, W. I., Smyth, J. T., Wedel, B., Boyles, R. R., Bird, G. S., Putney, J. W., Jr. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 281: 24979-24990, 2006.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,570 Da
NCBI Official Full Name
Protein orai-2
NCBI Official Synonym Full Names
ORAI calcium release-activated calcium modulator 2
NCBI Official Symbol
ORAI2
NCBI Official Synonym Symbols
CBCIP2; C7orf19; MEM142B; TMEM142B
NCBI Protein Information
protein orai-2; H_NH0514P08.8; putative protein ORAI2-2; transmembrane protein 142B; CAP-binding protein complex interacting protein 2; CAP-binding protein complex-interacting protein 2
UniProt Protein Name
Protein orai-2
Protein Family
UniProt Gene Name
ORAI2
UniProt Synonym Gene Names
C7orf19; CBCIP2; TMEM142B
UniProt Entry Name
ORAI2_HUMAN
Uniprot Description
TMEM142B: Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel subunit which mediates Ca(2+) influx and increase in Ca(2+)- selective current by synergy with the Ca(2+) sensor, STIM1. Belongs to the Orai family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: growth cone; integral to membrane
Molecular Function: protein binding
Research Articles on ORAI2
Similar Products
Product Notes
The ORAI2 orai2 (Catalog #AAA1753764) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Orai2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Orai2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the ORAI2 orai2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Orai2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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