Rat anti-Mouse CD8a Monoclonal Antibody | anti-CD8a antibody

Anti-Mouse CD8a Monoclonal Antibody (Alexa Fluor 647 Conjuaged)

Cat. Num. AAA2556908

• Gene Names: Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD8a; Monoclonal Antibody; Anti-Mouse CD8a Monoclonal Antibody (Alexa Fluor 647 Conjuaged); T-cell surface glycoprotein CD8 alpha chain; CD8A; T-lymphocyte differentiation antigen T8/Leu-2; MAL; anti-CD8a antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 53.6.7
• Form/Format: Liquid; PBS with 0.05% Proclin300, 1% BSA
• Sequence Length: 247

• Applicable Applications for anti-CD8a antibody: Flow Cytometry (FC/FACS)
• Conjugation: Alexa Fluor 647
• Preparation and Storage: Store at 2-8 degree C and protected from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(C57BL/6 murine splenocytes are stained with Anrti-Mouse CD8a Monoclonal Antibody(Alexa Fluor 647 Conjuaged)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

Related Product Information for anti-CD8a antibody

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked alpha and beta chains that form the alpha(CD8a)/beta(CD8b) heterodimer and alpha/alpha homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 alpha/beta heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR alpha/beta T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Zamoyska R. 1994. Immunity 1:243. 3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

NCBI and Uniprot Product Information

• NCBI GI #: 124487437
• NCBI GeneID: 12525
• NCBI Accession #: NP_001074579.1
• NCBI GenBank Nucleotide #: NM_001081110.2
• UniProt Accession #: P01731
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8 antigen, alpha chain
• NCBI Official Symbol: Cd8a
• NCBI Official Synonym Symbols: Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: Cd8a
• UniProt Synonym Gene Names: Lyt-2; Lyt2

Uniprot Description

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.