Rabbit anti-Human DBH Polyclonal Antibody | anti-DBH antibody

DBH Antibody (Center)

Cat. Num. AAA9203907

• Gene Names: DBH; DBM
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DBH; Polyclonal Antibody; DBH Antibody (Center); Dopamine beta-hydroxylase; Dopamine beta-monooxygenase; Soluble dopamine beta-hydroxylase; anti-DBH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DBH antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 199-232 amino acids from the Central region of human DBH.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 617

• Applicable Applications for anti-DBH antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysate from SH-SY5Y cell line, using DBH Antibody (Center). MBS9203907 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)

Related Product Information for anti-DBH antibody

Conversion of dopamine to noradrenaline.

Product Categories/Family for anti-DBH antibody

Crown Antibodies; Cancer; Metabolism; Neuroscience

References

Humphray S.J.,et al.Nature 429:369-374(2004).
Lamouroux A.,et al.EMBO J. 6:3931-3937(1987).
Kobayashi K.,et al.Nucleic Acids Res. 17:1089-1102(1989).
Li B.,et al.Biochem. J. 313:57-64(1996).
Liu T.,et al.J. Proteome Res. 4:2070-2080(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 116534900
• NCBI GeneID: 1621
• NCBI Accession #: NP_000778.3
• NCBI GenBank Nucleotide #: NM_000787.3
• UniProt Accession #: P09172; Q5T381; Q96AG2
• Molecular Weight: 69065
• NCBI Official Full Name: dopamine beta-hydroxylase
• NCBI Official Synonym Full Names: dopamine beta-hydroxylase (dopamine beta-monooxygenase)
• NCBI Official Symbol: DBH
• NCBI Official Synonym Symbols: DBM
• NCBI Protein Information: dopamine beta-hydroxylase
• UniProt Protein Name: Dopamine beta-hydroxylase
• Protein Family: Dopamine beta-hydroxylase
• UniProt Gene Name: DBH
• UniProt Entry Name: DOPO_HUMAN

NCBI Description

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008]

Uniprot Description

DBH: Conversion of dopamine to noradrenaline. Homotetramer composed of two non-covalently bound disulfide-linked dimers. Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids. Belongs to the copper type II ascorbate-dependent monooxygenase family.

Protein type: Cell cycle regulation; Amino Acid Metabolism - tyrosine; Oxidoreductase; Apoptosis; EC 1.14.17.1; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: membrane; cytoplasm; extracellular region; integral to membrane

Molecular Function: copper ion binding; L-ascorbic acid binding; dopamine beta-monooxygenase activity; catalytic activity

Biological Process: fear response; dopamine catabolic process; maternal behavior; response to amphetamine; cytokine production; locomotory behavior; response to pain; leukocyte mediated immunity; behavioral response to ethanol; glucose homeostasis; memory; regulation of cell proliferation; norepinephrine biosynthetic process; synaptic transmission; catecholamine biosynthetic process; homoiothermy; positive regulation of vasoconstriction; visual learning; blood vessel remodeling; leukocyte migration

Disease: Dopamine Beta-hydroxylase Deficiency, Congenital