Western Blot (WB)
Rabbit anti-Human, Mouse CD73 Polyclonal Antibody | anti-CD73 antibody
Anti-CD73 antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Peptide affinity purified
Synonyms
CD73; Polyclonal Antibody; Anti-CD73 antibody; Polyclonal Anti- CD73; anti-CD73 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Peptide affinity purified
Form/Format
Liquid; 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.
Applicable Applications for anti-CD73 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC)
Application Notes
WB (1:1000-2000)
IHC/ICC (1:50-1:200)
FC (1:50-1:100)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
IHC/ICC (1:50-1:200)
FC (1:50-1:100)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to the region of CD73.
Preparation and Storage
Store at 4°C after thawing (1 week).
Aliquot and store at -20°C for long term (at least one year).
Avoid repeated freeze and thaw cycles
Aliquot and store at -20°C for long term (at least one year).
Avoid repeated freeze and thaw cycles
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-CD73 antibody
5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene.1 CD73 or ecto-5'-nucleotidase (5'-NT) is a widely expressed ecto-enzyme which catalyzes the dephosphorylation of AMP and other nucleoside monophosphates. CD73 participates in purine salvage through this enzymatic activity, supplying cells with precursors for energy metabolism and nucleic acid biosynthesis. As an enzyme that produces adenosine, CD73 can also regulate adenosine receptor engagement in many tissues. In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells2
References
1. Misumi Y, Ogata S, Ohkubo K, Hirose S, Ikehara Y (August 1990). "Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form". Eur. J. Biochem. 191 (3): 563-9. 2. Resta R, Yamashita Y, Thompson LF (1998). "Ecto-enzyme and signaling functions of lymphocyte CD73.". Immunol. Rev. 161: 95-109
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Product Notes
The CD73 (Catalog #AAA355206) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD73 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD73 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC). WB (1:1000-2000) IHC/ICC (1:50-1:200) FC (1:50-1:100) Other applications have not been tested. The optimal dilutions should be determined by end user. Researchers should empirically determine the suitability of the CD73 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD73, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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