Western Blot (WB)
Rabbit CD133 Polyclonal Antibody | anti-CD133 antibody
Anti-CD133 antibody
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified
Synonyms
CD133; Polyclonal Antibody; Anti-CD133 antibody; Polyclonal Anti- CD133; anti-CD133 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified
Form/Format
Liquid
Applicable Applications for anti-CD133 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Application Notes
WB (1:1000)
IHC-P (1:200)
ICC (1:200)
FC (1:100-200)
Other applications have not been tested.
The optimal dilutions should be determined by end user
IHC-P (1:200)
ICC (1:200)
FC (1:100-200)
Other applications have not been tested.
The optimal dilutions should be determined by end user
Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide
Immunogen
Recombinant human CD133.
Preparation and Storage
Store at 4°C after thawing (1 week).Aliquot and store at -20°C for long term (atleast one year).Avoid repeated freeze and thaw cycles.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-CD133 antibody
Background/Introduction: CD133, originally known as AC133.1 CD133 is a glycoprotein also known in humans and rodents as Prominin 1 (PROM1).2 It is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. Most of the PROM1 gene is contained in 23 exons distributed over more than 50 kb of genomic sequence.3 CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, various pediatric brain tumors, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, testes, and some other cell types. CD133-positive tumor cells represent the cellular population that confers glioma radioresistance and could be the source of tumor recurrence after radiation. Targeting DNA damage checkpoint response in cancer stem cells may overcome this radioresistance and provide a therapeutic model for malignant brain cancers4.
References
1. Yin A, et al (1997). "AC133, is a novel marker for human hematopoietic stem and progenitor cells". Blood 90 (12): 5002-5012. 2. Corbeil D, Fargeas C, Huttner W (2001). "Rat prominin, like its mouse and human orthologues, is a pentaspan membrane glycoprotein". Biochem Biophys Res Commun 285 (4): 939-44. 3. Maw, M. A., Corbeil, D., Koch, J., Hellwig, A., Wilson-Wheeler, J. C., Bridges, R. J., Kumaramanickavel, G., John, S., Nancarrow, D., Roper, K., Weigmann, A., Huttner, W. B., Denton, M. J. A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration. Hum. Molec. Genet. 9: 27-34, 2000.4. Bao, S., Wu, Q., McLendon, R. E., Hao, Y., Shi, Q., Hjelmeland, A. B., Dewhirst, M. W., Bigner, D. D., Rich, J. N. Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. Nature 444: 756-760, 2006.
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Product Notes
The CD133 (Catalog #AAA355164) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD133 antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD133 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). WB (1:1000) IHC-P (1:200) ICC (1:200) FC (1:100-200) Other applications have not been tested. The optimal dilutions should be determined by end user. Researchers should empirically determine the suitability of the CD133 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD133, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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