Rabbit anti-Human CD73 Polyclonal Antibody | anti-NT5E antibody

Anti-CD73 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104317

• Gene Names: NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: CD73; Polyclonal Antibody; Anti-CD73 Antibody; Rabbit Polyclonal; CD73/NT5E Antibody; Rabbit PAb; Antigen Affinity Purified; 5'-nucleotidase; ecto (CD73); Anti-CALJA Antibody; Anti-E5NT Antibody; Anti-eN Antibody; Anti-eNT Antibody; Anti-NT Antibody; Anti-NT5 Antibody; Anti-NTE Antibody; anti-NT5E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CD73/NT5E
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NT5E antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Human CD73/NT5E protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD73/NT5E (rh CD73/NT5E). CD73/NT5E specific IgG was purified by Human CD73/NT5E affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NT5E rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:63 kDaObserved band size:63 kDa)

Related Product Information for anti-NT5E antibody

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo-and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.

Product Categories/Family for anti-NT5E antibody

Phosphatases and Regulators

References

Resta R. et al., 1997, Cell Signal. 9 (2): 131-9. Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90. Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55. Grunewald JK. et al., 2010, J Inflamm. 7 (1): 10.

NCBI and Uniprot Product Information

• NCBI GI #: 4505467
• NCBI GeneID: 4907
• NCBI Accession #: NP_002517.1
• NCBI GenBank Nucleotide #: NM_002526.3
• UniProt Accession #: P21589; O75520; Q5W116; B3KQI8
• Molecular Weight: 57,949 Da
• NCBI Official Full Name: 5'-nucleotidase isoform 1 preproprotein
• NCBI Official Synonym Full Names: 5'-nucleotidase, ecto (CD73)
• NCBI Official Symbol: NT5E
• NCBI Official Synonym Symbols: NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• NCBI Protein Information: 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: NT5E
• UniProt Synonym Gene Names: NT5; NTE; 5'-NT
• UniProt Entry Name: 5NTD_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries