Rabbit anti-Human, Mouse CD73 Polyclonal Antibody | anti-CD73 antibody

CD73 (NT5E), CT (NT5E, NT5, NTE, 5'-nucleotidase, Ecto-5'-nucleotidase, CD73) (MaxLight 550)

Cat. Num. AAA6283452

• Gene Names: NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CD73; Polyclonal Antibody; CD73 (NT5E); CT (NT5E; NT5; NTE; 5'-nucleotidase; Ecto-5'-nucleotidase; CD73) (MaxLight 550); NT5E; anti-CD73 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CD73. Species Crossreactivity: mouse
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-CD73 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 520-550 in the C-terminal region of human CD73, UniProt Accession #P21589.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of 033537 in mouse brain tissue lysate. CD73 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western Blot analysis of NT5E (arrow) using 033537. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.)

Testing Data

(All lanes: 033537 (1:1000) Lane 1: HepG2 whole cell lysate Lane 2: U266B1 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 63kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot analysis of lysates from U266B1, HepG2 cell line, mouse brain tissue lysate (from left to right at 20ug/lane), using 033537 (1:2000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:10,000 dilution was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human Placenta tissue reacted with 033537, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.)

Product Categories/Family for anti-CD73 antibody

Antibodies; CD Markers

References

1. Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003). 2. Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002). 3. Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990). 4. Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988). 5. Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4505467
• NCBI GeneID: 4907
• NCBI Accession #: NP_002517
• NCBI GenBank Nucleotide #: NM_002526.3
• UniProt Accession #: P21589; O75520; Q5W116; B3KQI8
• Molecular Weight: 57,949 Da
• NCBI Official Full Name: 5'-nucleotidase isoform 1 preproprotein
• NCBI Official Synonym Full Names: 5'-nucleotidase ecto
• NCBI Official Symbol: NT5E
• NCBI Official Synonym Symbols: NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• NCBI Protein Information: 5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: NT5E
• UniProt Synonym Gene Names: NT5; NTE; 5'-NT
• UniProt Entry Name: 5NTD_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries