Testing Data
(Peptide-+)
anti-Human, Mouse PHCA Antibody | anti-PHCA antibody
PHCA Antibody
Gene Names
ACER3; APHC; PHCA; PLDECO
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
PHCA; PHCA Antibody; Alkaline phytoceramidase; Aphc; Alkaline ceramidase; EC 3.5.1.-; Alkaline dihydroceramidase SB89; APHC; anti-PHCA antibody
Reactivity
Human, Mouse
Specificity
PHCA antibody detects endogenous levels of total PHCA protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
267
Applicable Applications for anti-PHCA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:40000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:40000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human PHCA.
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
(Peptide-+)
Testing Data
(Peptide-+)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human placenta tissue using PHCA antibody. Western blot analysis of extracts from COLO cells, using PHCA antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human placenta tissue using PHCA antibody. Western blot analysis of extracts from COLO cells, using PHCA antibody.)
Immunofluorescence (IF)
(Peptide-+ Immunofluorescence analysis of MCF-7 cells, using PHCA antibody.)
Immunofluorescence (IF)
(Peptide-+ Immunofluorescence analysis of MCF-7 cells, using PHCA antibody.)
Product Categories/Family for anti-PHCA antibody
References
Mao C., J. Biol. Chem. 276:26577-26588(2001). Li N., Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases. Ota T., Nat. Genet. 36:40-45(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,895 Da
NCBI Official Full Name
alkaline ceramidase 3 isoform a
NCBI Official Synonym Full Names
alkaline ceramidase 3
NCBI Official Symbol
ACER3
NCBI Official Synonym Symbols
APHC; PHCA; PLDECO
NCBI Protein Information
alkaline ceramidase 3
UniProt Protein Name
Alkaline ceramidase 3
Protein Family
UniProt Gene Name
ACER3
UniProt Synonym Gene Names
APHC; PHCA; AlkCDase 3; Alkaline CDase 3; aPHC
Uniprot Description
Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.
Research Articles on PHCA
Similar Products
Product Notes
The PHCA acer3 (Catalog #AAA854409) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The PHCA Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PHCA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000. Researchers should empirically determine the suitability of the PHCA acer3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHCA, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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