Western Blot (WB)
(Western Blot analysis of CD66b in A431 and HepG2 cell lysates in order using CD66b Rabbit pAb at dilution 1/500)
Rabbit anti-Human CD66b Polyclonal Antibody | anti-CD66b antibody
CD66b Rabbit pAb
Gene Names
CEACAM8; CD67; CGM6; CD66b; NCA-95
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
CD66b; Polyclonal Antibody; CD66b Rabbit pAb; CEA cell adhesion molecule 8; CD67; CGM6; NCA-95; anti-CD66b antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.8mg/ml (varies by lot)
Applicable Applications for anti-CD66b antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
IHC-P: 1:50-1:100
IHC-P: 1:50-1:100
Immunogen
Fusion protein of human CEACAM8
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western Blot analysis of CD66b in A431 and HepG2 cell lysates in order using CD66b Rabbit pAb at dilution 1/500)
Western Blot (WB)
(Western Blot analysis of CD66b in A431 and HepG2 cell lysates in order using CD66b Rabbit pAb at dilution 1/500)
Immunohistochemistry (IHC)
(Immunohistochemistry of CD66b in paraffin-embedded Human cervical cancer tissue using CD66b Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm and Cell membrane)
Immunohistochemistry (IHC)
(Immunohistochemistry of CD66b in paraffin-embedded Human cervical cancer tissue using CD66b Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm and Cell membrane)
Related Product Information for anti-CD66b antibody
Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:8776764, PubMed:2022629, PubMed:11590190). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:8776764, PubMed:2022629, PubMed:11590190). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764). By probing white blood cell cDNA libraries with tumor nonspecific crossreacting antigen, Arakawa et al. (1990) isolated a cDNA encoding CEACAM8, which they called NCA-W272. The deduced 349-amino acid protein contains 11 potential N-glycosylation sites and 4 cysteines expected to form 2 intramolecular disulfide linkages. It also has a signal peptide and several domains similar to those of other CEA family proteins, such as CEACAM1 (109770). Immunoblot analysis showed expression of a 70-kD protein that was reduced to a 37-kD major protein and a 34-kD minor protein in the presence of a glycosylation inhibitor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,154 Da
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 8
NCBI Official Synonym Full Names
carcinoembryonic antigen related cell adhesion molecule 8
NCBI Official Symbol
CEACAM8
NCBI Official Synonym Symbols
CD67; CGM6; CD66b; NCA-95
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 8
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 8
UniProt Gene Name
CEACAM8
UniProt Synonym Gene Names
CGM6
Uniprot Description
Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:8776764, PubMed:2022629, PubMed:11590190). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:8776764, PubMed:2022629, PubMed:11590190). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764).
Research Articles on CD66b
Similar Products
Product Notes
The CD66b ceacam8 (Catalog #AAA4758783) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD66b Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD66b can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC-P: 1:50-1:100. Researchers should empirically determine the suitability of the CD66b ceacam8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD66b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
