Rabbit anti-Human CD63 Polyclonal Antibody | anti-CD63 antibody

CD63, CT (CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, CD63) (MaxLight 550)

Cat. Num. AAA6283364

• Gene Names: CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CD63; Polyclonal Antibody; CT (CD63; MLA1; TSPAN30; CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; CD63) (MaxLight 550); anti-CD63 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CD63.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-CD63 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: IHC-P: Formalin-fixed, paraffin-embedded sections. Requires HIER using sodium citrate buffer pH6.0 prior to staining.; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 163-190 in the C-terminal region of human CD63.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB435 cell line lysates (35ug/lane) usingMBS643973. This demonstrates that MBS643973 detected the CD63 protein (arrow).)

Western Blot (WB)

(Western Blot using MBS643973 (1:2000) Lane 1: A2058 whole cell lysates Lane 2: A375 whole cell lysates Lane 3: HL-60 whole cell lysates Lane 4: THP-1 whole cell lysates Lane 5: U-87 MG whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit at 10,000. Predicted band size: 25kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of CD63 in formalin fixed paraffin embedded.human melanoma tissue sections usingMBS643973. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5hr at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with MBS643973 (1:25) for 1hr at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma using MBS643973 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS643973 for immunohistochemistry.)

Product Categories/Family for anti-CD63 antibody

Antibodies; CD Markers

References

Weng, J., et al. J. Virol. 83(15):7467-7474(2009). Kassahn, D., et al. Cell Death Differ. 16(1):115-124(2009). Logozzi, M., et al. PLoS ONE 4 (4), E5219 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4502679
• NCBI GeneID: 967
• NCBI Accession #: NP_001771.1
• NCBI GenBank Nucleotide #: NM_001780.5
• UniProt Accession #: P08962; Q5TZP3; Q8N6Z9; Q9UCG6; F8VZE2
• Molecular Weight: 17,271 Da
• NCBI Official Full Name: CD63 antigen isoform A
• NCBI Official Synonym Full Names: CD63 molecule
• NCBI Official Symbol: CD63
• NCBI Official Synonym Symbols: MLA1; ME491; LAMP-3; OMA81H; TSPAN30
• NCBI Protein Information: CD63 antigen; tspan-30; granulophysin; tetraspanin-30; melanoma-associated antigen MLA1; CD63 antigen (melanoma 1 antigen); melanoma-associated antigen ME491; ocular melanoma-associated antigen; lysosomal-associated membrane protein 3; lysosome-associated
• UniProt Protein Name: CD63 antigen
• Protein Family: CD63 antigen
• UniProt Gene Name: CD63
• UniProt Synonym Gene Names: MLA1; TSPAN30; LAMP-3; Tspan-30

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Uniprot Description

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.