Rabbit anti-Human CD3EAP Polyclonal Antibody | anti-CD3EAP antibody

CD3EAP polyclonal antibody

Cat. Num. AAA3001457

• Gene Names: CD3EAP; ASE1; CAST; ASE-1; PAF49
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: CD3EAP; Polyclonal Antibody; CD3EAP polyclonal antibody; ASE1; CAST; PAF49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CD3E-associated protein; RNA polymerase I-associated factor PAF49; anti-CD3EAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: CD3EAP Polyclonal Antibody detects endogenous levels of CD3EAP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-CD3EAP antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
• Application Notes: WB: 1/500-1/2000; IHC: 1/100-1/300; ELISA: 1/40,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human CD3EAP. AA range: 441-490
• Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
• Function: Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
• PTM: Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.; Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.; Phosphorylated upon DNA damage, probably by ATM or ATR.
• Similarity: Belongs to the eukaryotic RPA34 RNA polymerase subunit family.
• Subcellular Location: Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
• Subunit: Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E.
• Miscellaneous: It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function.
• Caution: It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling.
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of A549 cells using CD3EAP Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).)

NCBI and Uniprot Product Information

• NCBI GI #: 6912246
• NCBI GeneID: 10849
• NCBI Accession #: NP_036231.1
• NCBI GenBank Nucleotide #: NM_012099.1
• UniProt Accession #: O15446; Q32N11; Q7Z5U2; Q9UPF6
• Molecular Weight: 54,986 Da
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA34
• NCBI Official Synonym Full Names: CD3e molecule, epsilon associated protein
• NCBI Official Symbol: CD3EAP
• NCBI Official Synonym Symbols: ASE1; CAST; ASE-1; PAF49
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA34; A34.5; antisense to ERCC 1; CD3E-associated protein; antisense to ERCC-1 protein; CD3 epsilon associated protein; CD3-epsilon-associated protein; RNA polymerase I-associated factor PAF49; CD3e antigen, epsilon
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA34
• UniProt Gene Name: CD3EAP
• UniProt Synonym Gene Names: ASE1; CAST; PAF49; ASE-1; CAST
• UniProt Entry Name: RPA34_HUMAN

Uniprot Description

ASE-1: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Belongs to the eukaryotic RPA34 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Transcription initiation complex

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleolus; RNA polymerase I transcription factor complex; chromosome; nucleus; DNA-directed RNA polymerase I complex

Molecular Function: DNA-directed RNA polymerase activity

Biological Process: negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transmembrane receptor protein tyrosine kinase signaling pathway; rRNA transcription