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Western Blot (WB) (Western blot (WB) analysis of CD322 polyclonal antibody at 1:500 dilutionLaneA: A549 whole cell lysateLaneB: HEK293T whole cell lysateLaneC: MEF whole cell lysate)

Rabbit anti-Human, Mouse CD322 Polyclonal Antibody | anti-CD322 antibody

CD322 polyclonal antibody

Gene Names
JAM2; JAMB; CD322; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Synonyms
CD322; Polyclonal Antibody; CD322 polyclonal antibody; Junctional adhesion molecule B; JAM-B; Junctional adhesion molecule 2; JAM-2; Vascular endothelial junction-associated molecule; VE-JAM; JAM2; C21orf43; VEJAM; UNQ219; PRO245; anti-CD322 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
CD322 polyclonal antibody detects endogenous levels of CD322 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD322 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:200
Immunogen
Synthetic peptide, corresponding to Human CD322
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of CD322 polyclonal antibody at 1:500 dilutionLaneA: A549 whole cell lysateLaneB: HEK293T whole cell lysateLaneC: MEF whole cell lysate)

Western Blot (WB) (Western blot (WB) analysis of CD322 polyclonal antibody at 1:500 dilutionLaneA: A549 whole cell lysateLaneB: HEK293T whole cell lysateLaneC: MEF whole cell lysate)
Related Product Information for anti-CD322 antibody
Junctional adhesion molecule (JAM) is a member of the immunoglobulin superfamily expressed in tight junctions of epithelial cells and endothelial cells. It is implicated in transendothelial migration of leukocytes. JAM is constitutively expressed on circulating monocytes, neutrophils, lymphocyte subsets and platelets. The JAM family consists of JAM-A, JAM-B and JAM-C, alternatively designated JAM-1, JAM-2 and JAM-3, respectively. JAM-A localizes with F-Actin at the cell-cell contacts and at the membrane ruffles. It is involved in cell to cell adhesion through homophilic interactions and plays a role in the organization of tight junctions and modulation of leukocyte extravasation. JAM-B interacts with discrete subsets of PBLs, suggesting that it may play a role in lymphocyte trafficking. JAM-B and JAM-C proteins are binding partners; JAM-C may be a functional JAM-B receptor. Specifically, JAM-B adheres to T cells through heterotypic interactions with JAM-C. The JAM-B/JAM-C interaction may play a role in T, NK and dendritic cellular inflammation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,207 Da
NCBI Official Full Name
junctional adhesion molecule B isoform 3
NCBI Official Synonym Full Names
junctional adhesion molecule 2
NCBI Official Symbol
JAM2
NCBI Official Synonym Symbols
JAMB; CD322; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43
NCBI Protein Information
junctional adhesion molecule B; JAM-2; JAM-IT/VE-JAM; vascular endothelial junction-associated molecule
UniProt Protein Name
Junctional adhesion molecule B
UniProt Gene Name
JAM2
UniProt Synonym Gene Names
C21orf43; VEJAM; JAM-B; JAM-2; VE-JAM
UniProt Entry Name
JAM2_HUMAN

NCBI Description

This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

JAM2: May play a role in the processes of lymphocyte homing to secondary lymphoid organs. Belongs to the immunoglobulin superfamily.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q21.2

Cellular Component: tight junction; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protein heterodimerization activity

Biological Process: extracellular matrix organization and biogenesis; cell-cell adhesion; negative regulation of cell adhesion; blood coagulation; leukocyte migration

Research Articles on CD322

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Product Notes

The CD322 jam2 (Catalog #AAA3014951) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD322 polyclonal antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD322 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500~1:1000 IHC: 1:50~1:200. Researchers should empirically determine the suitability of the CD322 jam2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD322, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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