Rabbit anti-Mouse CD316 Polyclonal Antibody | anti-CD316 antibody

CD316, ID (Immunoglobulin superfamily member 8, CD81 partner 3, Glu-Trp-Ile EWI motif-containing protein 2, EWI-2, Keratinocytes-associated transmembrane protein 4, KCT-4, LIR-D1, IGSF8, CD81P3, EWI2, KCT4) (Azide free) (HRP)

Cat. Num. AAA6309611

• Gene Names: IGSF8; EWI2; PGRL; CD316; EWI-2; KCT-4; CD81P3; LIR-D1
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD316; Polyclonal Antibody; ID (Immunoglobulin superfamily member 8; CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; EWI-2; Keratinocytes-associated transmembrane protein 4; KCT-4; LIR-D1; IGSF8; CD81P3; EWI2; KCT4) (Azide free) (HRP); KCT4; Immunoglobulin superfamily member 8; Prostaglandin regulatory-like protein; anti-CD316 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CD316 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: IGSF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-262 amino acids from the Central region of human IGSF8.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(IGSF8 Antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the IGSF8 antibody detected the IGSF8 protein (arrow).)

Immunohistochemistry (IHC)

(IGSF8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGSF8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-CD316 antibody

Antibodies; Prostaglandins

References

Kolesnikova, T.V., et al. Neoplasia 11(1):77-86(2009). Rocha-Perugini, V., et al. PLoS ONE 3 (4), E1866 (2008): Kettner, S., et al. Mol. Cell. Biol. 27(21):7718-7726(2007). Sala-Valdes, M., et al. J. Biol. Chem. 281(28):19665-19675(2006). Yang, X.H., et al. J. Biol. Chem. 281(18):12976-12985(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 16445029
• NCBI GeneID: 93185
• NCBI Accession #: NP_443100.1
• NCBI GenBank Nucleotide #: NM_052868.6
• UniProt Accession #: Q969P0; Q8NG09; Q96DP4; Q9BTG9
• Molecular Weight: 40,191 Da
• NCBI Official Full Name: immunoglobulin superfamily member 8
• NCBI Official Synonym Full Names: immunoglobulin superfamily member 8
• NCBI Official Symbol: IGSF8
• NCBI Official Synonym Symbols: EWI2; PGRL; CD316; EWI-2; KCT-4; CD81P3; LIR-D1
• NCBI Protein Information: immunoglobulin superfamily member 8
• UniProt Protein Name: Immunoglobulin superfamily member 8
• UniProt Gene Name: IGSF8
• UniProt Synonym Gene Names: CD81P3; EWI2; KCT4; IgSF8; EWI-2; KCT-4; PGRL

NCBI Description

This gene encodes a member the EWI subfamily of the immunoglobulin protein superfamily. Members of this family contain a single transmembrane domain, an EWI (Glu-Trp-Ile)-motif and a variable number of immunoglobulin domains. This protein interacts with the tetraspanins CD81 and CD9 and may regulate their role in certain cellular functions including cell migration and viral infection. The encoded protein may also function as a tumor suppressor by inhibiting the proliferation of certain cancers. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.