Chicken Platelet endothelial cell adhesion molecule-1 ELISA Kit | PECAM-1/CD31 elisa kit

Chicken Platelet endothelial cell adhesion molecule-1 ELISA Kit

Cat. Num. AAA743761

• Gene Names: PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• Reactivity: Chicken
• Synonyms: Platelet endothelial cell adhesion molecule-1; Chicken Platelet endothelial cell adhesion molecule-1 ELISA Kit; PECAM-1/CD31 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This PECAM-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken PECAM-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PECAM-1/CD31 elisa kit

Principle of the Assay: PECAM-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PECAM-1 antibody and an PECAM-1-HRP conjugate. The assay sample and buffer are incubated together with PECAM-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PECAM-1 concentration since PECAM-1 from samples and PECAM-1-HRP conjugate compete for the anti-PECAM-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PECAM-1 from the sample, fewer sites are left to bind PECAM-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PECAM-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PECAM-1/CD31 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 313760624
• NCBI GeneID: 5175
• NCBI Accession #: NP_000433.4
• NCBI GenBank Nucleotide #: NM_000442.4
• Molecular Weight: 81,378 Da
• NCBI Official Full Name: platelet endothelial cell adhesion molecule
• NCBI Official Synonym Full Names: platelet/endothelial cell adhesion molecule 1
• NCBI Official Symbol: PECAM1
• NCBI Official Synonym Symbols: CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• NCBI Protein Information: platelet endothelial cell adhesion molecule; CD31 antigen
• UniProt Protein Name: Platelet endothelial cell adhesion molecule
• UniProt Gene Name: PECAM1
• UniProt Synonym Gene Names: PECAM-1
• UniProt Entry Name: PECA1_HUMAN

NCBI Description

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Uniprot Description

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; cell adhesion; signal transduction; blood coagulation; leukocyte migration; phagocytosis