Rabbit CD31 Polyclonal Antibody | anti-CD31 antibody

CD31, CT (Pecam1, Pecam, Pecam-1, Platelet endothelial cell adhesion molecule, CD31) (AP)

Cat. Num. AAA6283060

• Gene Names: Pecam1; Cd31; Pecam; C85791; PECAM-1
• Reactivity: Hamster, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD31; Polyclonal Antibody; CT (Pecam1; Pecam; Pecam-1; Platelet endothelial cell adhesion molecule; CD31) (AP); Pecam1; anti-CD31 antibody

• Host: Rabbit
• Reactivity: Hamster, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CD31 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse CD31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 511-540 amino acids from the C-terminal region of human Mouse CD31.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse CD31 Antibody (C-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the MouseCD31 antibody detected the Mouse CD31 protein (arrow).)

Western Blot (WB)

(Mouse CD31 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the MouseCD31 antibody detected the MouseCD31 protein (arrow).)

Related Product Information for anti-CD31 antibody

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Modulates BDKRB2 activation (By similarity). Induces susceptibility to atherosclerosis.

Product Categories/Family for anti-CD31 antibody

Antibodies; CD Markers

References

DeLisser, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(43):18616-18621(2010). Zhang, C., et al. Dev. Dyn. 239(10):2594-2602(2010). Enciso, J.M., et al. Dev. Dyn. 239(10):2570-2583(2010). Ni, A., et al. Dev. Dyn. 239(9):2354-2366(2010). Sessa, A., et al. Genes Dev. 24(16):1816-1826(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 74024907
• NCBI GeneID: 18613
• NCBI Accession #: NP_032842.2; NP_001027550.1; NP_113779.1
• NCBI GenBank Nucleotide #: NM_008816.3
• UniProt Accession #: P16284; Q08481
• Molecular Weight: 80kDa; 76.2kDa
• NCBI Official Full Name: platelet endothelial cell adhesion molecule isoform 1
• NCBI Official Synonym Full Names: platelet/endothelial cell adhesion molecule 1
• NCBI Official Symbol: Pecam1
• NCBI Official Synonym Symbols: Cd31; Pecam; C85791; PECAM-1
• NCBI Protein Information: platelet endothelial cell adhesion molecule
• UniProt Protein Name: Platelet endothelial cell adhesion molecule
• UniProt Gene Name: PECAM1
• UniProt Synonym Gene Names: PECAM-1
• UniProt Entry Name: PECA1_HUMAN

Uniprot Description

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; blood coagulation; signal transduction; cell adhesion; leukocyte migration; phagocytosis